Hu Bing, Zhao Jie-Yu, Nie Yong, Qin Xiao-Yu, Zhang Kai-Duan, Xing Jian-Min, Wu Xiao-Lei
Group of Biochemical Engineering, Department of Chemical Engineering, College of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102401, China.
Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology of China, Beijing 102401, China.
Microorganisms. 2021 Sep 29;9(10):2054. doi: 10.3390/microorganisms9102054.
Utilization of low-cost, environmental-friendly microbial enhanced oil recovery (MEOR) techniques in thermal recovery-processed oil reservoirs is potentially feasible. However, how exogenous microbes facilitate crude oil recovery in this deep biosphere, especially under mesophilic conditions, is scarcely investigated. In this study, a thermal treatment and a thermal recurrence were processed on crude oil collected from Daqing Oilfield, and then a 30-day incubation of the pretreated crude oil at 37 °C was operated with the addition of two locally isolated hydrocarbon-degrading bacteria, DQS3-9A1 and sp. DQ12-45-1b, respectively. The pH, surface tension, hydrocarbon profiles, culture-dependent cell densities and taxonomies, and whole and active microbial community compositions were determined. It was found that both DQS3-9A1 and sp. DQ12-45-1b successfully induced culture acidification, crude oil bioemulsification, and residual oil sub-fraction alteration, no matter whether the crude oil was thermally pretreated or not. Endogenous bacteria which could proliferate on double heated crude oil were very few. Compared with , sp. was substantially more effective at inducing the proliferation of varied species in one-time heated crude oil. Meanwhile, the effects of sp. on crude oil bioemulsification and hydrocarbon profile alteration were not significantly influenced by the ploidy increasing of NaCl contents (from 5 g/L to 50 g/L), but the reconstructed bacterial communities became very simple, in which the genus was predominant. Our study provides useful information to understand MEOR trials on thermally processed oil reservoirs, and proves that this strategy could be operated by using the locally available hydrocarbon-degrading microbes in mesophilic conditions with different salinity degrees.
在热采油藏中利用低成本、环境友好的微生物强化采油（MEOR）技术具有潜在可行性。然而，外源微生物如何在这个深部生物圈中促进原油采收，尤其是在嗜温条件下，却鲜有研究。在本研究中，对从大庆油田采集的原油进行了热处理和热循环处理，然后分别添加两种本地分离的烃降解菌DQS3-9A1和sp. DQ12-45-1b，在37℃下对预处理后的原油进行了30天的培养。测定了pH值、表面张力、烃类分布、基于培养的细胞密度和分类学，以及完整和活跃的微生物群落组成。结果发现，无论原油是否经过热预处理，DQS3-9A1和sp. DQ12-45-1b都成功诱导了培养物酸化、原油生物乳化和残余油亚组分改变。能在二次加热原油上增殖的内源细菌很少。与[未提及的对比对象]相比，sp. 在诱导一次加热原油中多种物种增殖方面效果显著更好。同时，sp. 对原油生物乳化和烃类分布改变的影响不受NaCl含量（从5 g/L增加到50 g/L）倍性增加的显著影响，但重建后的细菌群落变得非常简单，其中[未提及的属名]属占主导。我们的研究为理解热采油藏的MEOR试验提供了有用信息，并证明该策略可以通过在不同盐度的嗜温条件下使用本地可得的烃降解微生物来实施。
