Li X W, Sun H C, Liu X H
Department of Endodontics, School of Stomatology, Jilin University, Changchun 130021, China.
Department of Pathology, School of Stomatology, Jilin University, Changchun 130021, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Jan 9;53(1):42-48. doi: 10.3760/cma.j.issn.1002-0098.2018.01.009.
To evaluate the effect of vascular endothelial growth factor (VEGF)-loaded microspheres on dental pulp tissue regeneration and vascularization . release experiment and human umbilical vein endothelial cell migration experiment were conducted with VEGF loaded microspheres. The dental pulp stem cells (DPSC) were co-cultured with VEGF microspheres to observe the compatibility between the cells and the microspheres. DPSC and VEGF loaded microspheres were injected into the root lumen through the apical foramen, which were then transplanted subcutaneously into nude mice. Histological and immunohistochemical features were observed after nine weeks. DPSCs attached and spread on the surface of the microspheres. HE staining showed that the regenerated pulp-like tissue fulfilled the whole apex and middle third of the root. Differentiated odontoblast-like cells aligned with the existing tubular root dentin. VEGF-loaded microspheres promoted the regeneration of pulp-like tissues and formation of blood vessels.
为评估负载血管内皮生长因子（VEGF）的微球对牙髓组织再生和血管化的影响，对负载VEGF的微球进行了释放实验和人脐静脉内皮细胞迁移实验。将牙髓干细胞（DPSC）与VEGF微球共培养，以观察细胞与微球之间的相容性。将DPSC和负载VEGF的微球通过根尖孔注入根管腔，然后皮下移植到裸鼠体内。九周后观察组织学和免疫组化特征。DPSC附着并铺展在微球表面。苏木精-伊红（HE）染色显示，再生的牙髓样组织充满了根尖和牙根中三分之一。分化的成牙本质细胞样细胞与现有的管状根牙本质排列一致。负载VEGF的微球促进了牙髓样组织的再生和血管形成。
