Division of Gynecologic Oncology, Princess Margaret Hospital Cancer Centre, Toronto, ON, Canada.
Lunenfeld-Tanenbaum Research Institute at Sinai Health Systems, Mt. Sinai Hospital, 60 Murray Street, 6-10016-3, Toronto, ON, M5T 3L9, Canada.
J Ovarian Res. 2018 Jul 4;11(1):56. doi: 10.1186/s13048-018-0428-6.
Primary cytoreductive surgery followed by adjuvant chemotherapy is the standard treatment for advanced epithelial ovarian cancer. The average interval between surgery and chemotherapy initiation is approximately 4-weeks at most centers; however, since surgery may accelerate residual tumor growth, a shorter interval may be more beneficial.
The murine ID8 cell model of ovarian cancer was used to examine the efficacy of cisplatin treatment administered perioperatively or 7 days after surgical wounding. Luciferase-expressing cells ID8 cells were injected intraperitoneally (i.p.) into female C57/Bl6 mice. Fourteen days post-injection, animals received an abdominal incision or anesthesia alone and received i.p. cisplatin either on the surgical day or 7 days later, or received no chemotherapy. Additional animals received cisplatin 28 days after wounding for comparison.
Abdominal tumor mass increased 2.5-fold in wounded vs. unwounded animals as determined by bioluminescent in vivo tumor imaging. Cisplatin administered on the day of wounding decreased tumor burden by 50%, as compared to 90% in unwounded animals. Cisplatin on day 7 or day 28 decreased tumor burden by 80 and 37% respectively.
Surgical wounding increases ovarian tumor mass and decreases perioperative cisplatin efficacy in this animal model. Administration of cisplatin 1 week after surgery was more effective than cisplatin administered perioperatively or 4 weeks after surgery.
初次细胞减灭术联合辅助化疗是治疗晚期上皮性卵巢癌的标准方法。大多数中心的手术和化疗起始之间的平均间隔时间约为 4 周；然而，由于手术可能加速残留肿瘤生长，因此较短的间隔时间可能更有益。
使用小鼠 ID8 卵巢癌细胞模型来研究围手术期或手术后 7 天给予顺铂治疗的疗效。将表达荧光素酶的 ID8 细胞经腹腔内（i.p.）注射到雌性 C57/Bl6 小鼠中。注射后 14 天，动物接受腹部切口或单独麻醉，并在手术当天或 7 天后接受 i.p.顺铂治疗，或不接受化疗。其他动物在创伤后 28 天接受顺铂治疗以进行比较。
通过体内肿瘤成像的生物发光确定，与未受伤的动物相比，受伤的动物腹部肿瘤质量增加了 2.5 倍。与未受伤的动物相比，在受伤当天给予顺铂可使肿瘤负担减少 50%，而在未受伤的动物中则减少 90%。在第 7 天或第 28 天给予顺铂可使肿瘤负担分别减少 80%和 37%。
在该动物模型中，手术创伤会增加卵巢肿瘤的体积，并降低围手术期顺铂的疗效。手术后 1 周给予顺铂比围手术期或手术后 4 周给予顺铂更有效。
