小鼠主要组织相容性复合体中dm1突变的分子基础：一个D/L杂交基因。
Molecular basis of the dm1 mutation in the major histocompatibility complex of the mouse: a D/L hybrid gene.
作者信息
Sun Y H, Goodenow R S, Hood L
出版信息
J Exp Med. 1985 Nov 1;162(5):1588-602. doi: 10.1084/jem.162.5.1588.
Abstract
The H-2dm1 mutation is unique among all described H-2 mutations in that two transplantation antigens, the H-2Dd and the H-2Ld, are affected. Here, we show that the mutant gene, Ddm1, is formed by fusion of the 5' part of the Dd gene and the 3' part of the Ld gene, with the region in between deleted. The recombination junction is located in the third exon, which encodes the alpha 2 region of the protein. When the hybrid gene is transfected into mouse L cells, serological and biochemical analyses indicate the Ddm1 antigen expressed in the transformant line is identical to the mutant molecule in dm1 spleen cells. These results demonstrate that the D/L hybrid gene is most likely responsible for the dm1 mutant phenotype.
摘要
H-2dm1突变在所有已描述的H-2突变中是独特的，因为两种移植抗原H-2Dd和H-2Ld受到影响。在此，我们表明突变基因Ddm1是由Dd基因的5'部分与Ld基因的3'部分融合形成的，中间区域缺失。重组连接点位于第三个外显子，该外显子编码蛋白质的α2区域。当将杂交基因转染到小鼠L细胞中时，血清学和生化分析表明转化细胞系中表达的Ddm1抗原与dm1脾细胞中的突变分子相同。这些结果表明D/L杂交基因很可能是dm1突变表型的原因。
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本文引用的文献
1
Immunochemical evidence in two haplotypes for at least three D region-encoded molecules, D, L, and R.在两个单倍型中存在至少三种D区域编码分子（D、L和R）的免疫化学证据。
J Immunol. 1981 May;126(5):1713-6.
2
DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.编码BALB/c小鼠Ld移植抗原的基因的DNA序列。
Science. 1982 Feb 5;215(4533):679-82. doi: 10.1126/science.7058332.
3
Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer.通过DNA介导的基因转移鉴定BALB/c H-2Ld基因。
Science. 1982 Feb 5;215(4533):677-9. doi: 10.1126/science.7058331.
4
The making of strand-specific M13 probes.链特异性M13探针的制备。
Gene. 1982 Mar;17(3):271-7. doi: 10.1016/0378-1119(82)90143-3.
5
History and genealogy of the H-2Kb mutants from the C57BL/6Kh colony.来自C57BL/6Kh群体的H-2Kb突变体的历史和谱系。
Immunogenetics. 1982;15(2):177-85. doi: 10.1007/BF00621950.
6
Biochemistry of the gene products from murine MHC mutants.小鼠主要组织相容性复合体（MHC）突变体基因产物的生物化学
Annu Rev Genet. 1980;14:241-77. doi: 10.1146/annurev.ge.14.120180.001325.
7
The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events.一个突变的H-2基因的结构表明，H-2基因多态性的产生可能是通过类似基因转换的事件发生的。
Nature. 1983 Feb 24;301(5902):671-4. doi: 10.1038/301671a0.
8
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J Immunol. 1983 Nov;131(5):2440-4.
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Proc Natl Acad Sci U S A. 1983 Jan;80(1):242-6. doi: 10.1073/pnas.80.1.242.
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