Evans G A, Margulies D H, Camerini-Otero R D, Ozato K, Seidman J G
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1994-8. doi: 10.1073/pnas.79.6.1994.
A genomic clone encoding H-2Ld, a mouse major transplantation antigen, has been identified and the structure of the H-2Ld gene has been partially determined. We isolated 35 genomic clones from a BALB/c (H-2d) genomic library by hybridization to mouse or human probes. One of these clones encodes H-2Ld as determined by two criteria. First, the gene encodes a protein that is identical at the 76 known amino acid positions for H-2Ld. Second, when introduced into L cells by DNA-mediated gene transfer, a new H-2 antigen is expressed that is recognized by anti-H-2Ld monoclonal antibodies. The sequence of the H-2Ld protein predicted by the DNA sequences shows more than 80% homology to known H-2 antigens. H-2L-like sequences are found in mutant H-2Kb molecules, suggesting that gene conversion or reciprocal recombination may play a role in the development of H-2 polymorphism.
一个编码小鼠主要移植抗原H-2Ld的基因组克隆已被鉴定，并且H-2Ld基因的结构已被部分确定。我们通过与小鼠或人类探针杂交，从BALB/c（H-2d）基因组文库中分离出35个基因组克隆。通过两个标准确定，其中一个克隆编码H-2Ld。首先，该基因编码的蛋白质在H-2Ld已知的76个氨基酸位置上是相同的。其次，当通过DNA介导的基因转移导入L细胞时，会表达一种新的H-2抗原，该抗原可被抗H-2Ld单克隆抗体识别。由DNA序列预测的H-2Ld蛋白质序列与已知的H-2抗原显示出超过80%的同源性。在突变的H-2Kb分子中发现了H-2L样序列，这表明基因转换或相互重组可能在H-2多态性的发展中起作用。
