Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, 210002, Nanjing, Jiangsu, China.
Department of Oncology, Guizhou Provincial People's Hospital, 550002, Guiyang, Guizhou, China.
Gene Ther. 2018 Jul;25(4):312-319. doi: 10.1038/s41434-018-0029-4. Epub 2018 Jul 4.
The FoxM1 transcription factor plays an important role in the progression of HCC. Therefore, it is necessary to study cell regulation of FoxM1. In this study, we determined the expression of miR-214 and it was inversely associated with FoxM1 protein level in HCC; and suppression of FoxM1 translation by miR-214 mimics. We found that miR-214 targeted the 3'untranslated region of FoxM1 mRNA. In addition, the study found that DLX1 was the direct target of FoxM1 in HCC. Downregulation of FoxM1 inhibits the proliferation, migration, and invasion of HCC cells by miR-214. These results indicate that miR-214 may be used as a completely new molecular target by influencing FoxM1 expression in HCC.
FoxM1 转录因子在 HCC 的进展中起着重要作用。因此，有必要研究 FoxM1 的细胞调控。在这项研究中，我们确定了 miR-214 的表达，它与 HCC 中的 FoxM1 蛋白水平呈负相关；并且 miR-214 模拟物抑制 FoxM1 翻译。我们发现 miR-214 靶向 FoxM1 mRNA 的 3'非翻译区。此外，研究还发现 DLX1 是 HCC 中 FoxM1 的直接靶标。FoxM1 的下调通过 miR-214 抑制 HCC 细胞的增殖、迁移和侵袭。这些结果表明，miR-214 可能通过影响 HCC 中 FoxM1 的表达而成为一种全新的分子靶点。
