Zhang Naili, Ma Lina, Liu Xiaowei, Jiang Xiaorui, Yu Zhenhai, Zhao Dongmei, Zhang Luping, Zhang Chunlei, Huang Fei
Department of Human Anatomy, School of Basic Medical Science, Binzhou Medical University, 346 Guanhai Road, Laishan, Yantai, 264003, Shandong, China.
Department of Diagnostics, School of Medicine, Binzhou Medical University, 346 Guanhai Road, Laishan, Yantai, 264003, Shandong, China.
Cell Tissue Bank. 2018 Dec;19(4):591-601. doi: 10.1007/s10561-018-9708-z. Epub 2018 Jul 5.
The demineralized bone matrix (DBM) putty is a traditional bone graft utilized to facilitate the repair and reconstruction of bone. Recent studies indicated the DBM putties with the various carriers were different in bone repairing ability. In order to prepare a kind of DBM putty with a good biocompatibility and bioactivity, the DBM gel was processed from the DBM and the feasibility as a carrier for the DBM putty was evaluated. After the bovine DBM gel was prepared, the BMPs content as well as the ability to promote osteogenic differentiation of MC3T3-E1 cells in vitro were investigated. Then the DBM putty was prepared and filled into the rat calvarial defect model to evaluate the bone repairing ability by micro-CT and histology. The result showed there was 2.953 ± 0.054 ng BMP contained in per gram of the DBM gel. And the ALP production of MC3T3-E1 cells in the DBM gels group increased with prolonged culturing, the mineralized nodules formed in MC3T3-E1 cells on 14th day after co-culture. The putty prepared by DBM gel was easy to handle without loss of DBM particles at room temperature. In the rat calvarial bone defect experiment, histological observation showed more mature bone formed in the DBM putty group than that in the type I collagen group at 12 weeks, which indicated the bone putty prepared by DBM gel exhibited a better bone repair capability.
脱矿骨基质（DBM）糊剂是一种用于促进骨修复和重建的传统骨移植材料。最近的研究表明，不同载体的DBM糊剂在骨修复能力上存在差异。为了制备一种具有良好生物相容性和生物活性的DBM糊剂，由DBM制备了DBM凝胶，并评估了其作为DBM糊剂载体的可行性。制备牛DBM凝胶后，研究了其骨形态发生蛋白（BMPs）含量以及体外促进MC3T3-E1细胞成骨分化的能力。然后制备DBM糊剂并填充到大鼠颅骨缺损模型中，通过显微CT和组织学评估骨修复能力。结果显示，每克DBM凝胶中含有2.953±0.054 ng BMP。DBM凝胶组MC3T3-E1细胞的碱性磷酸酶（ALP）产量随培养时间延长而增加，共培养14天后MC3T3-E1细胞形成矿化结节。由DBM凝胶制备的糊剂在室温下易于操作，且不会损失DBM颗粒。在大鼠颅骨缺损实验中，组织学观察显示，在12周时，DBM糊剂组形成的骨比I型胶原组更成熟，这表明由DBM凝胶制备的骨糊剂具有更好的骨修复能力。
