Abe Hiroko, Takei Chikako, Sakakura Motoshi, Yajima Daisuke, Iwase Hirotato
Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chiba-shi, Chiba-ken, Japan.
BioChromato, Inc., Fujisawa-shi, Kanagawa-ken, Japan.
Methods Mol Biol. 2018;1810:115-124. doi: 10.1007/978-1-4939-8579-1_11.
Rapid and accurate analysis of drugs using direct analysis in real time-mass spectrometry (DART-MS) combined with thermal desorption pyrolysis (TDP/DART-MS) is described in this chapter. Rapid drug screening is very important in cases of suspected drug abuse. However, acquiring accurate analytical data requires highly optimized sample handling and technical expertise. Although rapid drug analysis using mass spectrometry provides reliable data, it is not commonly used because of its complexity. Thus, a comprehensive analysis system for drugs that requires minimal optimization of sample preparation and analytical conditions is strongly desired. DART-MS involves ambient ionization mass spectrometric analysis with little to no sample preparation. We have coupled this approach with thermal TDP/DART-MS and demonstrate how it can be used to detect drugs in complex matrices such as urine or blood.
本章介绍了结合热脱附热解（TDP/DART-MS）的实时直接分析质谱法（DART-MS）对药物进行快速准确的分析。在疑似药物滥用的情况下，快速药物筛查非常重要。然而，获取准确的分析数据需要高度优化的样品处理和专业技术知识。尽管使用质谱进行快速药物分析可提供可靠的数据，但由于其复杂性，并不常用。因此，迫切需要一种对样品制备和分析条件要求最少优化的药物综合分析系统。DART-MS涉及几乎无需样品制备的常压离子化质谱分析。我们将这种方法与热TDP/DART-MS相结合，并展示了它如何用于检测尿液或血液等复杂基质中的药物。
