College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China.
Academy of Agricultural Sciences, Industrial Crops Institute of Hubei, Wuhan 430064, China.
Int J Mol Sci. 2022 Oct 31;23(21):13296. doi: 10.3390/ijms232113296.
A lobed leaf is a common trait in plants, but it is very rare in Lauraceae plants, including species of In the study of germplasm resources of , we found lobed leaf variant seedlings, and the variation could be inherited stably. Studying the lobed leaf mechanism of var. can offer insight into the leaf development mechanism of woody plants. RNA-seq and small RNA-seq analysis results showed that a total of 8091 differentially expressed genes (DEGs) and 16 differentially expressed miRNAs were identified in var. . Considering previous research results, a leaf margin morphological development related miRNA, pne-miRNA319a, was primary identified as a candidate miRNA. Target gene prediction showed that a total of 2070 genes were predicted to be the target genes of differentially expressed miRNAs. GO enrichment analysis of differentially expressed target genes suggested that is related to lobed leaf formation. The TRV-VIGS gene silencing of led to lobed leaves in seedlings. The downregulation of led to lobed leaves. The yeast two-hybrid test and bimolecular fluorescence complementation test confirmed that the PnTCP2 protein interacted with the PnLBD41 protein. Based on the expression analysis of gene-silenced leaves and RNA-seq and small RNA-seq analysis results, pne- miRNA319a and might also play important roles in this process. In conclusion, plays an important and vital role in the formation of the lobed leaves of var.
复叶是植物的一个常见特征，但在樟科植物中，包括 属的物种中，这一特征非常罕见。在 属植物种质资源研究中，我们发现了复叶变异苗，这种变异可以稳定遗传。研究 var. 的复叶机制可以深入了解木本植物的叶片发育机制。RNA-seq 和 small RNA-seq 分析结果表明，在 var. 中总共鉴定到 8091 个差异表达基因（DEGs）和 16 个差异表达 miRNA。考虑到先前的研究结果，我们初步鉴定出一个与叶片边缘形态发育相关的 miRNA，pne-miRNA319a，作为候选 miRNA。靶基因预测表明，总共预测到 2070 个基因是差异表达 miRNA 的靶基因。差异表达靶基因的 GO 富集分析表明，与复叶形成有关。TRV-VIGS 基因沉默导致 幼苗出现复叶。下调 导致复叶。酵母双杂交试验和双分子荧光互补试验证实了 PnTCP2 蛋白与 PnLBD41 蛋白相互作用。基于基因沉默叶片的表达分析以及 RNA-seq 和 small RNA-seq 分析结果，pne-miRNA319a 和 可能也在这个过程中发挥重要作用。综上所述， 在 var. 的复叶形成中发挥着重要而关键的作用。
