University of Konstanz, Department of Chemistry, Universitätsstrasse 10, Konstanz 78457, Germany.
Analyst. 2018 Aug 20;143(17):4040-4050. doi: 10.1039/c8an00706c.
Analytical ultracentrifugation is a powerful technique for analyzing particles in solution, and has proved valuable for a wide range of applications in chemistry, biochemistry and material sciences for many years. The field is presently seeing a resurgence of instrument development from commercial and academic groups. To date, no modern optical modeling techniques have ever been applied to the basic imaging properties of the optical system in analytical ultracentrifugation. In this manuscript we provide a contextual framework for the application of such techniques, including an overview of the essential optical principles. The existing commercial and open source detection systems are evaluated for imaging performance, highlighting the limitations of chromatic aberration for broadband acquisitions. These results are the inspiration for a new mirror-based design, free of chromatic aberration. Our findings present a path forward for continued development in imaging and detector technology, where improved data quality will now push the limits of detection and resolution of analytical ultracentrifugation for years to come.
分析超速离心是一种用于分析溶液中颗粒的强大技术，多年来已被证明在化学、生物化学和材料科学的广泛应用中具有价值。目前，商业和学术团体正在重新开发仪器。迄今为止，没有现代光学建模技术应用于分析超速离心的基本光学系统的成像特性。在本文中，我们为这些技术的应用提供了一个背景框架，包括对基本光学原理的概述。评估了现有的商业和开源检测系统的成像性能，突出了宽带采集时色差的局限性。这些结果为无色差的新型基于反射镜的设计提供了灵感。我们的研究结果为成像和探测器技术的持续发展提出了一个方向，未来提高数据质量将推动分析超速离心检测和分辨率的极限。
