芯片上糖模拟文库与 C 型凝集素的筛选揭示了结构特征，这些特征负责 Dectin-2 相对于 DC-SIGN/R 和 langerin 的优先结合。
On-Chip Screening of a Glycomimetic Library with C-Type Lectins Reveals Structural Features Responsible for Preferential Binding of Dectin-2 over DC-SIGN/R and Langerin.
机构信息
Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133, Milano, Italy.
Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, 38000, Grenoble, France.
出版信息
Chemistry. 2018 Sep 25;24(54):14448-14460. doi: 10.1002/chem.201802577. Epub 2018 Sep 3.
A library of mannose- and fucose-based glycomimetics was synthesized and screened in a microarray format against a set of C-type lectin receptors (CLRs) that included DC-SIGN, DC-SIGNR, langerin, and dectin-2. Glycomimetic ligands able to interact with dectin-2 were identified for the first time. Comparative analysis of binding profiles allowed their selectivity against other CLRs to be probed.
合成了一系列基于甘露糖和岩藻糖的聚糖模拟物库，并以微阵列的形式对一组 C 型凝集素受体（CLRs）进行了筛选，其中包括 DC-SIGN、DC-SIGNR、 langerin 和 dectin-2。首次鉴定出能够与 dectin-2 相互作用的聚糖模拟配体。对结合谱的比较分析允许探测它们对其他 CLRs 的选择性。
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