Massignam Eloísa T, Ferreira Maitê, Sanguinet Eduardo, Dupont Ágata, Klamt Fábio, Frantz Nilo, Bos-Mikich Adriana
Department of Biochemistry, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Department of Morphological Sciences, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
JBRA Assist Reprod. 2018 Sep 1;22(3):199-204. doi: 10.5935/1518-0557.20180044.
The present study analyzed the quality of bovine ovarian tissue after vitrification in a metal closed chamber, in terms of putative changes in tissue viability (lactate dehydrogenase -LDH- release), anti-oxidant defenses, and redox parameters caused by cryopreservation.
Small and large fragmented bovine ovarian tissue specimens were vitrified in a metal chamber. After rewarming, tissue samples were fixed or cultured for 48 hours. Glutathione (GSH), protein sulfhydryl content, Total Radical Trapping Antioxidant Potential (TRAP), and lactate dehydrogenase were analyzed immediately after rewarming and after tissue culture.
No changes in antioxidant parameters or viability of rewarmed tissue samples were found immediately or 48h after vitrification. The method of vitrification in a metal closed chamber used in this study preserved the quality of bovine ovarian tissue. Furthermore, our data showed that the size of the tissue specimens did not affect post-vitrification biochemical viability parameters.
We believe that the vitrification methodology employed in the present study is safe and effective, and should be evaluated for use in humans.
本研究分析了在金属密闭腔室中玻璃化冷冻后的牛卵巢组织质量，涉及冷冻保存引起的组织活力（乳酸脱氢酶-LDH-释放）、抗氧化防御及氧化还原参数的假定变化。
将大小不同的碎片化牛卵巢组织样本在金属腔室中进行玻璃化冷冻。复温后，组织样本进行固定或培养48小时。复温后及组织培养后立即分析谷胱甘肽（GSH）、蛋白质巯基含量、总自由基捕获抗氧化能力（TRAP）和乳酸脱氢酶。
玻璃化冷冻后即刻及48小时后，复温组织样本的抗氧化参数或活力均未发现变化。本研究中使用的金属密闭腔室玻璃化冷冻方法保存了牛卵巢组织的质量。此外，我们的数据表明组织样本的大小不影响玻璃化冷冻后的生化活力参数。
我们认为本研究采用的玻璃化冷冻方法安全有效，应评估其在人体中的应用。
