1Department of Pediatrics, University Federico II, Naples, Italy.
2Department of Sciences for Health Promotion and Mother and Child Care, University of Palermo, Italy.
J Med Microbiol. 2018 Aug;67(8):1090-1095. doi: 10.1099/jmm.0.000787. Epub 2018 Jul 5.
Bile is a hepatobiliary lipid-rich sterile solution, and its colonization by microorganisms defines the condition of bactibilia. In this study, we aimed to assess the bile microbiological flora and its potential link with comorbidity in women.
We performed a microbiologic investigation on 53 female patients with biliopancreatic diseases who granted consent, and we analysed the data using a MATLAB platform.
We found that the most frequent disease associated with bactibilia was pancreas head carcinoma (PHC) (P=0.0015), while the least frequent disease was gall bladder carcinoma (GBC) (P=0.0002). The most common microorganisms were Pseudomonas spp. (P<0.0001) and Escherichia coli (P<0.0001). In particular Pseudomonas spp. and E. coli were negatively correlated to PHC presence and positively correlated to CCA by both univariate and multivariate analysis.
Gram-negative bacteria have been linked to a tumour-associated inflammatory status. In the last 30 years, the analysis of mortality rate in Italy for PHC and GBC shows an increasing and a decreasing trend, respectively. Although this study targeted only 53 patients and does not reflect the frequency of diagnosis in a Southern Italian population, the decrease in GBC may raise the suggestion ofnon-adherence to a Mediterranean diet that may have become more prevalent in Southern Italy since the 1990s.
胆汁是一种富含肝胆汁的无菌溶液，其微生物定植定义了细菌胆汁的条件。本研究旨在评估女性胆汁微生物菌群及其与合并症的潜在联系。
我们对 53 名同意的胆胰疾病女性患者进行了微生物学调查，并使用 MATLAB 平台分析了数据。
我们发现与细菌胆汁相关的最常见疾病是胰头癌（PHC）（P=0.0015），而最不常见的疾病是胆囊癌（GBC）（P=0.0002）。最常见的微生物是假单胞菌属（P<0.0001）和大肠杆菌（P<0.0001）。特别是假单胞菌属和大肠杆菌通过单变量和多变量分析与 PHC 的存在呈负相关，与 CCA 呈正相关。
革兰氏阴性菌与肿瘤相关的炎症状态有关。在过去的 30 年中，意大利 PHC 和 GBC 死亡率的分析显示出上升和下降的趋势。尽管本研究仅针对 53 名患者，不能反映意大利南部人群的诊断频率，但 GBC 的减少可能表明非遵守地中海饮食的情况更为普遍，自 20 世纪 90 年代以来，意大利南部地区的地中海饮食更为普遍。
