Boggs D R, Malloy E, Boggs S S, Chervenick P A, Lee R E
J Lab Clin Med. 1977 Jan;89(1):80-92.
A transplantable murine breast carcinoma in mice was associated with marked leukemoid reaction. Within 1 week of subcutaneous implantation of tumor the leukocyte count began to increase and reached average levels of 165,000 leukocytes per cubic millimeter within 18 days. This represented an increase in mature neutrophils primarily, although other blood leukocytes were modestly increased as well. The total number of neutrophils per humerus was increased but no increase was detected in the number of myloblasts, promyelocytes, or myelocytes. The tritiated thymidine-labeling index of the latter three cells was not significantly changed during tumor growth. The number of progenitor cells forming granulocytic and mononuclear cells in vitro was decreased in the marrow during tumor growth. Colony-stimulating activity in plasma was slightly increased during the early phase of tumor growth and decreased during later phases. Emergence time of blood neutrophils was normal, as measured by labeling with tritiated thymidine, but decline in labeled cells was abnormally slow in tumor-bearing mice. There was a shift of erythropoiesis to the spleen, but total erythropoiesis appeared to be normal in most mice. Surgical excision of the tumor resulted in prompt reversal of the leukemoid reaction. In the aggregate these results are consistent with a hypothesis that the leukemoid reaction was the result of increased blood transit time of neutrophils primarily, rather than increased neutrophil production.
一种可移植的小鼠乳腺癌与显著的类白血病反应相关。在肿瘤皮下植入1周内，白细胞计数开始增加，18天内达到平均每立方毫米165,000个白细胞的水平。这主要表现为成熟中性粒细胞的增加，尽管其他血液白细胞也有适度增加。每只肱骨的中性粒细胞总数增加，但早幼粒细胞、中幼粒细胞或晚幼粒细胞的数量未检测到增加。在肿瘤生长过程中，后三种细胞的氚标记胸腺嘧啶核苷标记指数没有显著变化。肿瘤生长期间，骨髓中形成粒细胞和单核细胞的祖细胞数量减少。肿瘤生长早期血浆中的集落刺激活性略有增加，后期则降低。用氚标记胸腺嘧啶核苷标记法测量，血液中性粒细胞的出现时间正常，但在荷瘤小鼠中，标记细胞的减少异常缓慢。红细胞生成转移至脾脏，但大多数小鼠的总红细胞生成似乎正常。手术切除肿瘤导致类白血病反应迅速逆转。总体而言，这些结果与一种假设一致，即类白血病反应主要是中性粒细胞血液转运时间增加的结果，而非中性粒细胞生成增加。
