Shimizu Dai, Saito Tomoko, Ito Shuhei, Masuda Takaaki, Kurashige Junji, Kuroda Yosuke, Eguchi Hidetoshi, Kodera Yasuhiro, Mimori Koshi
Department of Surgery, Kyushu University Beppu Hospital, Tsurumihara, Japan.
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
Cancer Genomics Proteomics. 2018 Jul-Aug;15(4):313-320. doi: 10.21873/cgp.20089.
Peritoneal dissemination (PD) is one of the most common causes of cancer-related mortality in gastric cancer (GC). We aimed to identify PD-associated genes and investigate their role in GC.
We identified FGFR1 as a putative PD-associated gene using a bioinformatics approach. The biological significance of FGFR1 in epithelial-to-mesenchymal transition (EMT) was evaluated according to the correlation with genes that participated in EMT and FGFR1 knockdown experiments. The associations between FGFR1 expression and the clinicopathological features were examined.
FGFR1 expression positively correlated with SNAI1, VIM and ZEB1 expression, and negatively correlated with CDH1 expression. Knockdown of FGFR1 suppressed the malignant phenotype of GC cells. High FGFR1 expression significantly correlated with the peritoneal lavage cytology and synchronous PD positivity as well as poor prognosis.
High FGFR1 expression was associated with PD via promotion of EMT and led to a poor prognosis of GC patients.
腹膜播散（PD）是胃癌（GC）患者癌症相关死亡的最常见原因之一。我们旨在鉴定与PD相关的基因，并研究它们在胃癌中的作用。
我们采用生物信息学方法将FGFR1鉴定为一个假定的与PD相关的基因。根据FGFR1与参与上皮-间质转化（EMT）的基因的相关性以及FGFR1基因敲低实验，评估FGFR1在上皮-间质转化中的生物学意义。检测FGFR1表达与临床病理特征之间的关联。
FGFR1表达与SNAI1、VIM和ZEB1表达呈正相关，与CDH1表达呈负相关。敲低FGFR1可抑制GC细胞的恶性表型。FGFR1高表达与腹腔灌洗细胞学检查、同步PD阳性以及预后不良显著相关。
FGFR1高表达通过促进EMT与PD相关，并导致GC患者预后不良。
