Velez Norida, Alvarado Maira, Parra-Giraldo Claudia Marcela, Sánchez-Quitian Zilpa Adriana, Escandón Patricia, Castañeda Elizabeth
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá 110931, Colombia.
Unidad de Investigación en Proteomica y Micosis Humanas, Grupo de Investigacion en Enfermedades Infecciosas, Dpto de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá 110231, Colombia.
J Fungi (Basel). 2018 Jul 5;4(3):82. doi: 10.3390/jof4030082.
Cryptococcosis is a potentially fatal opportunistic mycosis that affects the lungs and central nervous system. It has been suggested that certain strains of may have the potential to be more virulent according to the molecular type. This study aims to investigate the association between virulence in the model and genotypic diversity of Colombian clinical and environmental isolates of . A total of 33 clinical and 12 environmental isolates were selected according to their geographical origin and sequence types (STs). Pathogenicity was determined using the model, and the cell and capsular size before and after inoculation was determined. For , virulence in revealed that death occurred on average on day 6 ( < 0.05) and that ST5C, 6C, 25C and 71C were the most virulent. In , death occurred at 7.3 days ( < 0.05), and ST47C, 58C, 75A and 106C were the most virulent. Capsular size increased for both species after passage in . In conclusion, the pathogenicity of strains in the invertebrate model is independent of molecular type or pathogenicity factor, even within the same ST, but it is possible to find variable degrees of pathogenicity.
隐球菌病是一种可能致命的机会性真菌病，可影响肺部和中枢神经系统。有研究表明，根据分子类型，某些隐球菌菌株可能具有更强的毒力。本研究旨在调查哥伦比亚临床和环境分离的隐球菌在果蝇模型中的毒力与基因型多样性之间的关联。根据地理来源和序列类型（STs）共选择了33株临床分离株和12株环境分离株。使用果蝇模型确定致病性，并测定接种前后的细胞和荚膜大小。对于新型隐球菌，果蝇模型中的毒力显示平均在第6天死亡（P<0.05），ST5C、6C、25C和71C毒力最强。对于格特隐球菌，在第7.3天死亡（P<0.05），ST47C、58C、75A和106C毒力最强。两种菌在果蝇传代后荚膜大小均增加。总之，在无脊椎动物果蝇模型中，隐球菌菌株的致病性与分子类型或致病因素无关，即使在同一ST内，但可能存在不同程度的致病性。
