Potential Mechanism of Action of Cyclosporin A in Human Dermal Fibroblasts-Transcriptomic Analysis of .

Effect of cyclosporin A (CsA) in a therapeutic concentration, on the expression of cytochrome P450 genes (), was investigated in normal human dermal fibroblast cells. The expression of 57 genes, encoding cytochrome P450 isoforms, was estimated using the microarray method. Amongst 396 normalized fluorescence signals related to cytochrome P450 activity, only 91 were strictly connected to and were analyzed using two methods: a self-organizing feature map of artificial neural networks and typical statistical analysis with significance level at ≤ 0.05. Comparing the samples from fibroblasts cultured with CsA and those cultured without, up-regulated changes of , , , , and down-regulated gene expression were observed. The mRNAs with increased changes were in the same neuron of the self-organizing feature map. All distinguished encode monooxygenases, which plays an important role in steroids biosynthesis and metabolism. Based on the obtained results, we can conclude that CsA in therapeutic concentration changes the expression profile of in human dermal fibroblasts, especially affecting genes linked to steroids synthesis and/or metabolism. It shows the potential mechanism of action of CsA in human dermal fibroblast cells.