Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (SA), Italy.
Institute of Crystallography of the National Council of Research (CNR), 00015 Monterotondo Scalo, Italy.
Sensors (Basel). 2018 Jul 5;18(7):2168. doi: 10.3390/s18072168.
In this work, the development of an impedimetric label-free immunosensor for the detection of O157:H7 is reported. Different immobilization techniques of monoclonal anti- were tested, in order to reach the very low limit of detections. The comparison between the immobilization procedures underlined the advantages of the oriented procedure and the use of a dendrimer, which allowed for immobilizing a higher number of antibody units, reaching a very high sensitivity. However, the use of activated ferrocene as electron-transferring mediator, which improved the electrical properties of the system, resulted in a very low limit of detection equal to 3 cfu/mL. This immunosensor was used to analyze milk and meat samples obtaining a good agreement with the results of the ELISA methods.
本工作报道了一种用于检测 O157:H7 的基于阻抗的无标记免疫传感器的研制。为了达到非常低的检测极限，我们测试了不同的单克隆抗体固定化技术。固定化程序的比较突出了定向程序和使用树状大分子的优势，这使得可以固定更多数量的抗体单位，从而达到非常高的灵敏度。然而，使用作为电子转移介质的活化二茂铁，改善了系统的电学性质，使检测极限达到 3 cfu/mL。该免疫传感器用于分析牛奶和肉类样品，与 ELISA 方法的结果具有良好的一致性。
