Department of Genetics & Molecular Biology in Botany, Institute of Botany, Christian-Albrechts-University at Kiel, Kiel, Germany.
Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, DK-6700, Esbjerg, Denmark.
Sci Rep. 2018 Jul 5;8(1):10187. doi: 10.1038/s41598-018-28473-z.
Marine Fungi are potent secondary metabolite producers. However, limited genetic information are available their biosynthetic gene clusters (BGCs) and their biotechnological applications. To overcome this lack of information, herein, we used next-generation sequencing methods for genome sequencing of two marine fungi, isolated from the German Wadden Sea, namely Calcarisporium sp. KF525 and Pestalotiopsis sp. KF079. The assembled genome size of the marine isolate Calcarisporium sp. KF525 is about 36.8 Mb with 60 BGCs, while Pestalotiopsis sp. KF079 has a genome size of 47.5 Mb harboring 67 BGCs. Of all BGCs, 98% and 97% are novel clusters of Calcarisporium sp. and Pestalotiopsis sp., respectively. Only few of the BGCs were found to be expressed under laboratory conditions by RNA-seq analysis. The vast majority of all BGCs were found to be novel and unique for these two marine fungi. Along with a description of the identified gene clusters, we furthermore present important genomic features and life-style properties of these two fungi. The two novel fungal genomes provide a plethora of new BGCs, which may have biotechnological applications in the future, for example as novel drugs. The genomic characterizations will provide assistance in future genetics and genomic analyses of marine fungi.
海洋真菌是具有强大次生代谢产物生产能力的微生物。然而，关于它们的生物合成基因簇（BGCs）及其生物技术应用的遗传信息有限。为了克服这一信息不足的问题，本文采用下一代测序方法对从德国瓦登海分离得到的两种海洋真菌，即 Calcarisporium sp. KF525 和 Pestalotiopsis sp. KF079，进行了基因组测序。海洋分离株 Calcarisporium sp. KF525 的组装基因组大小约为 36.8Mb，包含 60 个 BGCs，而 Pestalotiopsis sp. KF079 的基因组大小为 47.5Mb，含有 67 个 BGCs。所有 BGCs 中，Calcarisporium sp. 和 Pestalotiopsis sp. 分别有 98%和 97%是新颖的簇。通过 RNA-seq 分析，仅发现少数 BGCs 在实验室条件下表达。这两个海洋真菌的绝大多数 BGCs 都是新颖且独特的。除了描述鉴定的基因簇外，我们还介绍了这两种真菌的重要基因组特征和生活方式特性。这两个新的真菌基因组提供了大量新的 BGCs，它们可能具有生物技术应用的潜力，例如作为新型药物。这些基因组特征将为未来海洋真菌的遗传学和基因组分析提供帮助。
