He Yuxuan, Zhang Yong, Li Hongyan, Zhang Hong, Li Zongshuai, Xiao Longfei, Hu Junjie, Ma Youji, Zhang Quanwei, Zhao Xingxu
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Dose Response. 2018 Jun 20;16(2):1559325818778633. doi: 10.1177/1559325818778633. eCollection 2018 Apr-Jun.
This study investigated the toxicity of heavy ion radiation to mice testis by microRNA (miRNA) sequencing and bioinformatics analyses. Testicular indices and histology were measured following enterocoelia irradiation with a 2 Gy carbon ion beam, with the testes exhibiting the most serious injuries at 4 weeks after carbon ion radiation (CIR) exposure. Illumina sequencing technology was used to sequence small RNA libraries of the control and irradiated groups at 4 weeks after CIR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses implicated differential miRNAs in the regulation of target genes involved in metabolism, development, and reproduction. Here, 8 miRNAs, including miR-34c-5p, miR-138, and 6 let-7 miRNA family members previously reported in testis after radiation, were analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to validate miRNA sequencing data. The differentially expressed miRNAs described here provided a novel perspective for the role of miRNAs in testis toxicity following CIR.
本研究通过微小RNA（miRNA）测序和生物信息学分析，研究了重离子辐射对小鼠睾丸的毒性。用2 Gy碳离子束进行腹腔照射后，测量睾丸指数和组织学情况，碳离子辐射（CIR）暴露后4周时睾丸损伤最为严重。采用Illumina测序技术对CIR后4周对照组和照射组的小RNA文库进行测序。基因本体论和京都基因与基因组百科全书通路分析表明，差异miRNA参与了对涉及代谢、发育和生殖的靶基因的调控。在此，通过定量逆转录-聚合酶链反应（qRT-PCR）分析了8种miRNA，包括之前报道的辐射后睾丸中的miR-34c-5p、miR-138以及6个let-7 miRNA家族成员，以验证miRNA测序数据。本文描述的差异表达miRNA为miRNA在CIR后睾丸毒性中的作用提供了新的视角。
