Xu Xue-Yu, Wu De, Xu Sheng-Yu, Che Lian-Qiang, Fang Zheng-Feng, Feng Bin, Li Jian, Wu Cai-Mei, Lin Yan
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Theriogenology. 2018 Oct 1;119:105-113. doi: 10.1016/j.theriogenology.2018.06.026. Epub 2018 Jul 3.
To elucidate the role of microRNA (miRNA) during early testicular development, we constructed three small RNA libraries from boar testes at three timepoints. Utilizing Solexa deep sequencing technology, over 12 million reads were measured. Of a total of 263 known miRNAs, 159 were co-expressed in all libraries. Read counts of the top 20 most abundant miRNAs accounted for more than 75% of total known miRNAs, and in all libraries, miR-10b was the most abundant. Analysis of higher fold changes in miRNAs (|log2_fold changes| > 4) revealed seven miRNAs that were involved in testes development (miR-381, miR-205, miR-217, miR-146a-5p, miR-187, miR-215, and miR-195). Among these, miR-146a-5p and miR-195 played an important role in 0-day-old (N0) and 28-day-old (N28) boars; miR-187 and miR-205 primarily regulated reproductive processes in N28 and 120-day-old (N120) boars; and miR-381, miR-217, and miR-215 participated in the modulation of testes development during all three timepoints. These miRNAs regulated testes cell proliferation and apoptosis at different stages. Furthermore, the predicted miRNA targets from the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these differentially expressed miRNAs likely regulated developmental processes via PI3K-AKT, mitogen-activated protein kinase, and other related signaling pathways. Our results present a genome-wide miRNA profile from the testes of different-aged boars and provide a useful theoretical basis for further studies on the role of miRNA regulation in testes development during early life.
为阐明微小RNA（miRNA）在睾丸早期发育过程中的作用，我们在三个时间点构建了来自公猪睾丸的三个小RNA文库。利用Solexa深度测序技术，共检测到超过1200万条 reads。在总共263个已知miRNA中，有159个在所有文库中共同表达。前20个表达量最高的miRNA的reads计数占已知miRNA总数的75%以上，并且在所有文库中，miR-10b表达量最高。对miRNA中较高倍数变化（|log2_倍数变化| > 4）的分析揭示了7个参与睾丸发育的miRNA（miR-381、miR-205、miR-217、miR-146a-5p、miR-187、miR-215和miR-195）。其中，miR-146a-5p和miR-195在0日龄（N0）和28日龄（N28）的公猪中起重要作用；miR-187和miR-205主要在N28和120日龄（N120）的公猪中调节生殖过程；miR-381、miR-217和miR-215在所有三个时间点都参与了睾丸发育的调节。这些miRNA在不同阶段调节睾丸细胞增殖和凋亡。此外，基因本体论和京都基因与基因组百科全书通路分析预测的miRNA靶标表明，这些差异表达的miRNA可能通过PI3K-AKT、丝裂原活化蛋白激酶和其他相关信号通路调节发育过程。我们的结果展示了不同年龄公猪睾丸的全基因组miRNA图谱，并为进一步研究miRNA调控在生命早期睾丸发育中的作用提供了有用的理论基础。
