Li Hongyan, He Yuxuan, Yan Jiawei, Zhao Qiuyue, Di Cuixia, Zhang Hong
Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Reprod Toxicol. 2016 Oct;65:148-158. doi: 10.1016/j.reprotox.2016.07.014. Epub 2016 Jul 25.
The toxicological mechanism of low sperm motility induced by iron ion radiation (IIR) was investigated in mice. Reproductive organ indices were measured following whole-body irradiation with a 2Gy iron ion beam. Two-dimensional gel electrophoresis, immunoblotting and immunofluorescence were used to analyze protein expression, and real-time polymerase chain reaction was performed to confirm altered gene expression. Reproductive organ indices and sperm motility were lowest 2 weeks after IIR. Sperm function changes via testis and cauda epididymis function were also determined at this time point. Sixteen differentially expressed proteins were identified in sperm 2 weeks after IIR. Bioinformatics analysis indicated that alpha-enolase (Eno1) may be important in the regulation of glycolysis in sperm, and Eno1 expression was correlated with sperm motility. Eno1 may be a potential marker for low sperm motility induced by IIR, and these results may provide a useful reference for changes in astronaut fertility during long space missions.
研究了铁离子辐射（IIR）诱导小鼠精子活力低下的毒理学机制。用2Gy铁离子束全身照射后测量生殖器官指数。采用二维凝胶电泳、免疫印迹和免疫荧光分析蛋白质表达，并进行实时聚合酶链反应以确认基因表达的改变。IIR后2周生殖器官指数和精子活力最低。此时还测定了通过睾丸和附睾尾功能引起的精子功能变化。IIR后2周在精子中鉴定出16种差异表达蛋白。生物信息学分析表明，α-烯醇化酶（Eno1）可能在精子糖酵解调节中起重要作用，且Eno1表达与精子活力相关。Eno1可能是IIR诱导精子活力低下的潜在标志物，这些结果可能为长期太空任务期间宇航员生育能力的变化提供有用参考。
