Panin L E, Kolosova I E
Probl Endokrinol (Mosk). 1985 May-Jun;31(3):69-73.
Cyclic nucleotides are universal intermediary agents of hormones in the target tissues, however mechanisms of the regulation of the cAMP and cGMP content in the cell are rather complex and still obscure. More data have appeared of late on the involvement of blood serum lipoproteins in the regulation of various intracellular processes including adenylate cyclase activity. Therefore the purpose of the study was to investigate the effect of adrenaline, hydrocortisone, glucagon, insulin and blood serum lipoproteins on the cAMP and cGMP content in rat liver surviving sections. An attempt was made to study a cooperative effect of the above hormones and lipoproteins of various classes. The results obtained have shown that adrenaline and glucagon raise the cAMP level in rat liver surviving sections. The effect of adrenaline is mediated by beta-adrenoreceptors. Insulin lowers an increases the level of cAMP in liver sections determined by the effect of glucagon. A decrease of the initial cAMP content in response to insulin occurs after a short lag period. High density lipoproteins (HDLP3) reduce the cAMP content in liver surviving sections. A cooperative effect of lipoproteins of a very low density and adrenaline (or hydrocortisone) in the regulation of the cAMP content in the rat liver was revealed. The cGMP content in rat liver surviving sections does not change under the influence of the above hormones and lipoproteins.
环核苷酸是激素在靶组织中的通用中介物，然而细胞中cAMP和cGMP含量的调节机制相当复杂且仍不清楚。近年来，出现了更多关于血清脂蛋白参与包括腺苷酸环化酶活性在内的各种细胞内过程调节的数据。因此，本研究的目的是研究肾上腺素、氢化可的松、胰高血糖素、胰岛素和血清脂蛋白对大鼠肝脏存活切片中cAMP和cGMP含量的影响。试图研究上述激素和不同类别脂蛋白的协同作用。所获得的结果表明，肾上腺素和胰高血糖素可提高大鼠肝脏存活切片中的cAMP水平。肾上腺素的作用是由β-肾上腺素能受体介导的。胰岛素降低并增加由胰高血糖素作用所决定的肝脏切片中的cAMP水平。胰岛素作用后，经过短暂的延迟期，初始cAMP含量会下降。高密度脂蛋白（HDLP3）可降低肝脏存活切片中的cAMP含量。揭示了极低密度脂蛋白与肾上腺素（或氢化可的松）在调节大鼠肝脏cAMP含量方面的协同作用。在上述激素和脂蛋白的影响下，大鼠肝脏存活切片中的cGMP含量没有变化。
