Ruoff H J
Eur J Pharmacol. 1977 Aug 15;44(4):349-54. doi: 10.1016/0014-2999(77)90309-0.
The effect of adrenergic stimulation and blockade on the concentration of cAMP and cGMP was studied in the rat gastric mucosa. Adrenaline (0.5-2.0 mg/kg) elevated the gastric mucosal cAMP and cGMP levels up to 100% in a dose-dependent manner. Blockade of the beta-adrenoceptors with 4 mg/kg propranolol suppressed the adrenaline effect on the cAMP level and increased the effect on the cGMP concentration up to 500%. The opposite effect was found under alpha-adrenoceptor blockade. Phenoxybenzamine, 4 mg/kg, prevented the adrenaline effect on the cGMP level and increased the effect on the cAMP concentration up to 300%. Predominant stimulation of the beta-adrenoceptors by isoproterenol or of the alpha-adrenoceptors by phenylephrine caused smaller changes in the cyclic nucleotide concentration than did adrenaline. Their effect was more pronounced when the non-stimulated receptor was blocked. The data indicate that the rat gastric mucosa contains alpha- and beta-adrenoceptors. The effect of adrenaline on the gastric mucosal cAMP level is mediated by beta-adrenoceptors and that on the cGMP level by alpha-adrenoceptors.
在大鼠胃黏膜中研究了肾上腺素能刺激和阻断对环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）浓度的影响。肾上腺素（0.5 - 2.0毫克/千克）以剂量依赖的方式使胃黏膜cAMP和cGMP水平升高达100%。用4毫克/千克普萘洛尔阻断β - 肾上腺素能受体可抑制肾上腺素对cAMP水平的作用，并使对cGMP浓度的作用增加达500%。在α - 肾上腺素能受体阻断时发现了相反的效果。4毫克/千克的酚苄明可阻止肾上腺素对cGMP水平的作用，并使对cAMP浓度的作用增加达300%。异丙肾上腺素对β - 肾上腺素能受体的主要刺激或去氧肾上腺素对α - 肾上腺素能受体的刺激所引起的环核苷酸浓度变化比肾上腺素小。当未受刺激的受体被阻断时，它们的作用更明显。数据表明大鼠胃黏膜含有α和β肾上腺素能受体。肾上腺素对胃黏膜cAMP水平的作用由β - 肾上腺素能受体介导，而对cGMP水平的作用由α - 肾上腺素能受体介导。
