Bioinformatics analysis of microarray data to explore the key genes involved in mutation-induced cataract.

AIM

To reveal the mechanisms of 4 () mutation-induced cataract.

METHODS

GSE22362, including 3 -null lens and 3 wild-type lens, was obtained from Gene Expression Omnibus database. After data preprocessing, the differentially expressed genes (DEGs) were identified using the limma package. Based on Database for Annotation, Visualization and Integrated Discovery (DAVID) tool, functional and pathway enrichment analyses were performed for the DEGs. Followed by protein-protein interaction (PPI) network was constructed using STRING database and Cytoscape software. Furthermore, the validated microRNA (miRNA)-DEG pairs were obtained from miRWalk2.0 database, and then miRNA-DEG regulatory network was visualized by Cytoscape software.

RESULTS

A total of 176 DEGs were identified in -null lens compared with wild-type lens. In the PPI network, FBJ osteosarcoma oncogene (FOS), early growth response 1 (EGR1) and heme oxygenase (decycling) 1 (HMOX1) had higher degrees and could interact with each other. Besides, and were among the top 10 miRNAs in the miRNA-DEG regulatory network. Additionally, could target in the regulatory network.

CONCLUSION

, and might function in the pathogenesis of mutation-induced cataract.