Li Yuanbin, Liu Shujun, Zhang Fenglan, Jiang Pengfei, Wu Xinyi, Liang Yan
Department of Ophthalmology, Qilu Hospital of Shandong University Jinan, People's Republic of China ; Department of Ophthalmology, Yantai Yuhuangding Hospital Yantai, People's Republic of China.
Department of Ophthalmology, Yantai Yuhuangding Hospital Yantai, People's Republic of China.
Int J Clin Exp Med. 2015 Feb 15;8(2):2405-10. eCollection 2015.
This study aimed to examine and analyze the expression levels of hsa-miR-15a and hsa-miR-16-1 in lens epithelial cells from patients with age-related cataract to understand better the roles of these microRNAs in the pathogenesis of this disease. Lens epithelial cells of 60 age-related cataract patients (including 20 with cortical cataracts, 20 with nuclear cataracts, and 20 with posterior subcapsular cataracts) and 20 normal patients were included in the study. Real-time PCR was used to detect the expression of hsa-miR-15a-5p, hsa-miR-15a-3p, hsa-miR-16-1-5p, and hsa-miR-16-1-3p. Expression of the target genes of these microRNAs, namely bcl-2 and mcl-1, was also evaluated. hsa-miR-15a-5p, hsa-miR-15a-3p, and hsa-miR-16-1-5p were expressed at low levels in normal lens epithelial cells but at significantly higher levels in corresponding cells of patients with cortical, nuclear, or posterior subcapsular cataracts (P < 0.01). hsa-miR-16-1-3p was expressed at relatively high levels in normal lens epithelial cells but significantly decreased expression, or none at all, was detected in cells of patients from each cataract group (P < 0.01). Concerning their target genes bcl-2 and mcl-1, expression was detectable in normal lens epithelial cells, but their levels were significantly decreased in cataract patients, irrespective of type (P < 0.01). Expression of hsa-miR-15a-5p, hsa-miR-15a-3p, and hsa-miR-16-1-5p rose in lens epithelial cells in the three types of age-related cataract, which may suppress the expression of the anti-apoptotic genes bcl-2 and mcl-1, thereby contributing to the pathogenesis of age-related cataract through apoptosis.
本研究旨在检测和分析年龄相关性白内障患者晶状体上皮细胞中hsa-miR-15a和hsa-miR-16-1的表达水平，以更好地了解这些微小RNA在该疾病发病机制中的作用。本研究纳入了60例年龄相关性白内障患者（包括20例皮质性白内障患者、20例核性白内障患者和20例后囊下白内障患者）的晶状体上皮细胞以及20例正常患者的晶状体上皮细胞。采用实时荧光定量PCR检测hsa-miR-15a-5p、hsa-miR-15a-3p、hsa-miR-16-1-5p和hsa-miR-16-1-3p的表达。还评估了这些微小RNA的靶基因bcl-2和mcl-1 的表达。hsa-miR-15a-5p、hsa-miR-15a-3p和hsa-miR-16-1-5p在正常晶状体上皮细胞中低表达，但在皮质性、核性或后囊下白内障患者的相应细胞中表达显著升高（P<0.01）。hsa-miR-16-1-3p在正常晶状体上皮细胞中表达相对较高，但在各白内障组患者的细胞中表达显著降低或未检测到表达（P<0.01）。关于它们的靶基因bcl-2和mcl-1，在正常晶状体上皮细胞中可检测到表达，但在白内障患者中其水平显著降低，与白内障类型无关（P<0.01）。在三种类型的年龄相关性白内障中，hsa-miR-15a-5p、hsa-miR-15a-3p和hsa-miR-16-1-5p在晶状体上皮细胞中的表达升高，这可能抑制抗凋亡基因bcl-2和mcl-1的表达，从而通过细胞凋亡促进年龄相关性白内障的发病机制。
