血小板裂解物促进持续性上皮缺损的再上皮化：一项初步研究。
Platelet lysate promotes re-epithelialization of persistent epithelial defects: a pilot study.
作者信息
Abu-Ameerh Mohammed A, Jafar Hanan D, Hasan Maram H, Al Bdour Muawyah D, Msallam Mohammed, Ababneh Osama H, Alhattab Dana M, Al-Kurdi Ban, Awidi Abdelhalim A, Awidi Abdalla S
机构信息
Department of Ophthalmology, School of Medicine, The University of Jordan, Amman, Jordan.
Cell Therapy Center, The University of Jordan, Amman, Jordan.
出版信息
Int Ophthalmol. 2019 Jul;39(7):1483-1490. doi: 10.1007/s10792-018-0968-1. Epub 2018 Jul 5.
PURPOSE
To study the use of autologous platelet lysate prepared in a standardized method for the healing of persistent corneal epithelial defects (PED).
STUDY DESIGN
Clinical and experimental investigation.
METHODS
In this prospective pilot study (ClinicalTrials.gov identifier NCT02979912), ten patients with a PED duration of a minimum 14 days were included. Autologous platelet lysate was prepared in a standardized methodology. Repeated freeze-thaw cycles were used to lyse the platelets. Patients were advised to apply the eye drops four times a day and were evaluated at baseline and on days 7, 14, 21, 28.
RESULTS
No adverse events were reported due to the use of undiluted autologous platelet lysate. A total of 70% of patients had complete re-epithelialization within 28 days. Of these, 40% healed within 14 days (effective group) and 30% within 28 days (partially effective group).
CONCLUSIONS
Undiluted autologous platelet lysate, prepared according to a standardized methodology, is a safe and effective adjunct therapy for the treatment of PED.
目的
研究采用标准化方法制备的自体血小板裂解液用于持续性角膜上皮缺损（PED）愈合的情况。
研究设计
临床和实验研究。
方法
在这项前瞻性初步研究（ClinicalTrials.gov标识符NCT02979912）中，纳入了10例PED持续时间至少14天的患者。采用标准化方法制备自体血小板裂解液。通过反复冻融循环来裂解血小板。建议患者每天滴眼4次，并在基线以及第7、14、21、28天进行评估。
结果
未报告因使用未稀释的自体血小板裂解液而出现不良事件。共有70%的患者在28天内实现完全上皮化。其中，40%在14天内愈合（有效组），30%在28天内愈合（部分有效组）。
结论
按照标准化方法制备的未稀释自体血小板裂解液是治疗PED的一种安全有效的辅助治疗方法。
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