Liang Yi, Wang Chaoxi, Chen Apeng, Zhu Lei, Zhang Jie, Jiang Pucha, Yue Qiaoxin, De Gejing
Laboratory Medicine College, Guangdong Medical University, Guangdong, People's Republic of China.
Department of Neurological Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
J Cell Commun Signal. 2019 Mar;13(1):75-84. doi: 10.1007/s12079-018-0476-0. Epub 2018 Jul 5.
Pygo2 as a Wnt signaling pathway component has been detected in multiple cancer types. In this study, we identified Pygo2 expression features by immunohistochemistry in 73 central nervous system tumor specimens, in comparison with 14 normal brain tissues and surrounding non-tumorous tissues of tumor. Our study indicated that 59% of the patient tumor specimens exhibited positive Pygo2-staining and increases intensity with the grade of malignancy, especially for WHO grade III and IV gliomas, was observed high level expression, compared with normal brain tissues. Five out of nine WHO grade III anaplastic astrocytomas and seven out of nine WHO grade IV glioblastomas showed Pygo2-positive staining. The analysis of Pygo2 gene expression by quantitative real-time PCR of additional ten fresh patient samples yielded similar results. Further studies performed with stable cell lines in vitro demonstrated that Pygo2 render cells higher proliferation rate, migration and anchorage-independent colony-forming ability in soft agar. Taken together, our studies suggest an important role of Pygo2 in brain tumor progression.
Pygo2作为Wnt信号通路的一个组成部分，已在多种癌症类型中被检测到。在本研究中，我们通过免疫组织化学方法，在73例中枢神经系统肿瘤标本中鉴定了Pygo2的表达特征，并与14例正常脑组织以及肿瘤周围的非肿瘤组织进行了比较。我们的研究表明，59%的患者肿瘤标本显示Pygo2染色呈阳性，并且随着恶性程度的增加而染色强度增强，尤其是对于世界卫生组织（WHO）III级和IV级胶质瘤，与正常脑组织相比，观察到其高水平表达。9例WHO III级间变性星形细胞瘤中有5例以及9例WHO IV级胶质母细胞瘤中有7例显示Pygo2阳性染色。通过对另外10例新鲜患者样本进行定量实时PCR分析Pygo2基因表达，也得到了类似的结果。在体外对稳定细胞系进行的进一步研究表明，Pygo2使细胞具有更高的增殖率、迁移能力以及在软琼脂中不依赖贴壁的集落形成能力。综上所述，我们的研究表明Pygo2在脑肿瘤进展中起重要作用。
