School of Materials Science and Engineering, University of Jinan, Jinan, 510632, People's Republic of China.
Mikrochim Acta. 2018 Jul 5;185(8):362. doi: 10.1007/s00604-018-2906-3.
A fluorometric assay is described for sulfide ions determination. It is based on the finding that the oxidation of the non-fluorescent substrate thiamine (TH) by Cu(II) in basic solution to form fluorescent thiochrome is inhibited by sulfide ions. This results in a decrease in fluorescence intensity which is proportional to the concentration of sulfide ions. Under the optimized conditions, the decrease in fluorescence, best measured at excitation/emission wavelengths of 370/440 nm, decreases linearly in the 0.03 to 2.5 μM sulfide ions concentration range. The detection limit is 20 nM. The method shows excellent selectivity over many potentially interfering ions and has been successfully applied to the determination of sulfide ions in spiked tap water, lake water and the synthetic wastewater samples. The method is time-saving and environmentally friendly, and in our perception shows a great potential in water quality inspection and environmental monitoring. Graphical abstract A fluorescent assay for sulfide ions detection is proposed based on its inhibitory effect on the oxidation of thiamine by Cu(II) ions.
一种用于测定硫化物离子的荧光分析方法。其原理是基于在碱性溶液中，Cu(II)将非荧光底物硫胺素（TH）氧化为具有荧光的噻嗪，而硫化物离子对此反应具有抑制作用。这导致荧光强度降低，其降低程度与硫化物离子的浓度成正比。在优化条件下，在 370/440nm 的激发/发射波长下，荧光强度的降低在 0.03 至 2.5μM 硫化物离子浓度范围内呈线性变化。检测限为 20nM。该方法对许多潜在干扰离子具有优异的选择性，并已成功应用于加标自来水、湖水和合成废水样品中硫化物离子的测定。该方法省时且环保，我们认为它在水质检查和环境监测方面具有很大的潜力。
