Department of Forensic Chemistry, Criminal Investigation Police University of China, Shenyang 110854, PR China.
Anal Chim Acta. 2018 Jun 20;1010:69-75. doi: 10.1016/j.aca.2018.01.020.
A novel "turn-on" fluorescence probe (HHC)-Cu for sulfide (S) was synthesized and characterized based on dihydroxyhemicyanine-Cu complex. The probe displayed high sensitivity and selectivity for S over other analytes, including biothiols, NaCN, reactive oxygen species (ROS) and reactive nitrogen species (RNS) in aqueous solution, which was attributed to the large association constant and the fast kinetics of precipitation of CuS. The probe (HHC)-Cu can be regenerated and reversibly detected with S with a remarkable red-fluorescent change by alternating the addition of S and Cu. Fluorescent spectra of (HHC)-Cu toward S showed a high selectivity, a good linearity, a low limit of detection at 0.12 μM, a rapid response time (less than 30 s), and a wide pH range of 7-10. Importantly, the probe was successfully applied to detect the low level of S in waste water samples, corrupt blood samples, and living cells.
一种新型的“turn-on”荧光探针（HHC）-Cu 被合成并用于检测硫化物（S），其基于二羟基半花青-Cu 配合物。该探针在水溶液中对 S 具有高灵敏度和选择性，优于其他分析物，包括生物硫醇、NaCN、活性氧（ROS）和活性氮（RNS），这归因于 CuS 的大缔合常数和快速沉淀动力学。探针（HHC）-Cu 可以通过交替添加 S 和 Cu 进行再生和可逆检测，S 的存在会引起明显的红色荧光变化。（HHC）-Cu 对 S 的荧光光谱表现出高选择性、良好的线性、低检测限（0.12μM）、快速响应时间（小于 30s）和宽 pH 值范围（7-10）。重要的是，该探针成功应用于检测废水样品、腐败血液样品和活细胞中的低水平 S。
