Lane B P, Habicht G S, Jasper G S
Am J Pathol. 1977 Jan;86(1):71-80.
Rat tracheal tissues were maintained in organ culture for 1 to 2 weeks and then implanted heterotopically into syngeneic and outbred hosts. The grafts and surrounding tissues were removed at regular intervals and examined by light and electron microscopy. Allogeneic grafts were consistently rejected, and a constant pattern of immunologic response was observed during rejection. Infiltration by mononuclear cells occurred initially in the subepithelial region. Subsequently, small lymphocytes migrated through the basal lamina and insinuated themselves between the differentiated epithelial cells. Larger cells resembling activated lymphocytes were also present in the intraepithelial infiltrate. Both type of lymphocytes had cellular processes in contact with epithelial cells, but there was no associated death of epithelial cells. Later, the infiltrate in some areas of the epithelium inclded dividing cells and other large cells exhibiting ultrastructural features suggesting blast transformation. Epithelial cell death was evident during this phase. The sequence of events may reflect a process of recognition and specific cytotoxic action by lymphocytes.
将大鼠气管组织进行器官培养1至2周，然后异位植入同基因和远交系宿主。定期取出移植物和周围组织，通过光镜和电镜检查。同种异体移植物始终被排斥，在排斥过程中观察到恒定的免疫反应模式。单核细胞浸润最初发生在皮下区域。随后，小淋巴细胞穿过基膜并潜入分化的上皮细胞之间。上皮内浸润中也存在类似活化淋巴细胞的较大细胞。两种类型的淋巴细胞都有与上皮细胞接触的细胞突起，但上皮细胞没有相关死亡。后来，上皮某些区域的浸润包括分裂细胞和其他表现出超微结构特征提示母细胞转化的大细胞。在此阶段上皮细胞死亡明显。这些事件的顺序可能反映了淋巴细胞识别和特异性细胞毒性作用的过程。
