Staindl O, Lametschwandtner A
HNO. 1979 Jul;27(7):221-6.
In sequence with an earlier publication on the results of heterograft tracheal transplants in animals, changes in the Formol-Cialit preserved heterogenous tracheal grafts were studied by light microscopy, and by scanning and transmission electron microscopy. Two main queries arise. Where does the graft respiratory epithelium come from, and what happens to the grafted tracheal cartilage? We found that in the epithelium of graft the number of ciliated cells decreased and that there was ultrastructure evidence of cilial destruction, the goblet cells increased in number, there was a loss of strict polarity of the respiratory epithelium, subepithelial accumulations of cells of probable epithelial origin appeared, and there was a subepithelial accumulation of lymphocytes just beneath the basal lamina. Resorption and transformation of the grafted tracheal cartilage into connective tissue was also observed. Hence, the following conclusions may be made. (1) Small tracheal defects can be corrected by Formol-Cialit preserved heterologous grafts. (2) The mucosa of the host trachea produces a functioning respiratory epithelium in the graft. (3) Tracheal grafts should not exceed 2 x 3 cm in size otherwise a new stenosis from resorption and transformation of grafted cartilage into connective tissue will occur.
与之前一篇关于动物异种移植气管结果的出版物相衔接，通过光学显微镜、扫描电子显微镜和透射电子显微镜研究了福尔马林-西阿利特保存的异种气管移植物的变化。出现了两个主要问题。移植物的呼吸上皮来自何处，移植的气管软骨会发生什么变化？我们发现，在移植物的上皮中，纤毛细胞数量减少，有纤毛破坏的超微结构证据，杯状细胞数量增加，呼吸上皮的严格极性丧失，出现了可能起源于上皮的细胞在基底膜下的积聚，并且在基底膜下方有淋巴细胞的上皮下积聚。还观察到移植的气管软骨吸收并转化为结缔组织。因此，可以得出以下结论。（1）小的气管缺损可以用福尔马林-西阿利特保存的异种移植物矫正。（2）宿主气管的黏膜在移植物中产生有功能的呼吸上皮。（3）气管移植物的大小不应超过2×3厘米，否则会因移植软骨吸收并转化为结缔组织而出现新的狭窄。
