Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
1st Department of Pediatrics, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
PLoS One. 2018 Jul 6;13(7):e0200391. doi: 10.1371/journal.pone.0200391. eCollection 2018.
Plasma creatinine and urea are commonly used markers of kidney function in both acute and chronic renal failure. The needed repeated blood collection is associated with pain, stress and might lead to infections. Saliva has the potential to be a non-invasive alternative diagnostic fluid. The use of saliva in clinical practice is limited, since many factors affect the concentration of salivary biomarkers. The aim of our study was to analyze salivary creatinine and urea in the animal models of acute and chronic renal disease. Bilateral nephrectomy and adenine nephropathy were induced in adult male mice. Both, plasma creatinine and urea were higher in animals with renal failure compared to controls. Salivary creatinine was higher by 81% and salivary urea by 43% in comparison to the control group, but only in animals with bilateral nephrectomy and not in adenine nephropathy. Our results indicate that the increase of salivary creatinine and urea depends on the experimental model of renal failure and its severity. Further studies are needed to monitor the dynamics of salivary markers of renal function and to reveal determinants of their variability.
血浆肌酐和尿素常用于急性和慢性肾衰竭的肾功能标志物。需要反复采血会引起疼痛和应激，还可能导致感染。唾液有可能成为一种非侵入性的替代诊断液。由于许多因素会影响唾液生物标志物的浓度，因此唾液在临床实践中的应用受到限制。我们的研究目的是分析急性和慢性肾病动物模型中的唾液肌酐和尿素。在成年雄性小鼠中诱导双侧肾切除术和腺嘌呤肾病。与对照组相比，肾衰竭动物的血浆肌酐和尿素均升高。与对照组相比，唾液肌酐升高 81%，唾液尿素升高 43%，但仅在双侧肾切除动物中升高，而在腺嘌呤肾病动物中不升高。我们的结果表明，唾液肌酐和尿素的增加取决于肾衰竭的实验模型及其严重程度。需要进一步研究来监测肾功能唾液标志物的动态变化，并揭示其变异性的决定因素。
