National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.
J Med Virol. 2018 Nov;90(11):1681-1686. doi: 10.1002/jmv.25253. Epub 2018 Aug 16.
Zika virus (ZIKV), dengue virus (DENV), chikungunya virus (CHIKV) and yellow fever virus (YFV) share the same mosquito vectors and have similar clinical manifestations early stage of infection. Therefore, simultaneously differentiating these viruses from each other is necessary. We developed a multiplex real-time reverse-transcriptase polymerase chain reaction (RT-PCR) assay for the differentiation of these four viruses in a single tube. The linear range was established by regression analysis, and the R value for each virus was ≥0.98, and the 95% lower limit of detection for each virus was as follows (copies/reaction): ZIKV-Asian, 9; ZIKV-Africa, 15; CHIKV, 11; DENV-1, 19; DENV-2, 13; DENV-3, 24; DENV-4, 36; and YFV, 17. Meanwhile, our multiplex real-time RT-PCR has a good consistency with the commercial singleplex assay. In summary, the developed assay can be effectively used for the diagnosis of ZIKV, DENV, CHIKV, and YFV infections.
寨卡病毒（ZIKV）、登革热病毒（DENV）、基孔肯雅热病毒（CHIKV）和黄热病毒（YFV）共用相同的蚊媒，且在感染的早期具有相似的临床症状。因此，有必要将这些病毒彼此区分开来。我们开发了一种多重实时逆转录聚合酶链反应（RT-PCR）检测方法，可在单个管中区分这四种病毒。通过回归分析确定线性范围，每个病毒的 R 值均≥0.98，每个病毒的 95%检测下限如下（拷贝/反应）：ZIKV-亚洲型，9；ZIKV-非洲型，15；CHIKV，11；DENV-1，19；DENV-2，13；DENV-3，24；DENV-4，36；YFV，17。同时，我们的多重实时 RT-PCR 与商业单重检测试剂盒具有良好的一致性。总之，所开发的检测方法可有效用于 ZIKV、DENV、CHIKV 和 YFV 感染的诊断。
