Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
J Virol Methods. 2019 Jun;268:53-55. doi: 10.1016/j.jviromet.2019.03.014. Epub 2019 Mar 28.
Although certain mosquito-borne virus, such as Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Yellow fever virus (YFV) and West Nile virus (WNV), are an important public health concern in those countries where transmitter mosquitoes are endemic, several cases of travelers from those endemic countries have been recently reported in Europe. Thus, early diagnosis of these viruses is essential for patient management and adoption of preventive measures. An assay for the simultaneous detection of DENV, CHIKV, ZIKV, YFV and WNV based on a multiplex real-time (RT)-PCR and its usefulness for diagnosis in infection screenings and surveillance of arbovirus in non-endemic countries are described.
虽然某些蚊媒病毒，如登革热病毒（DENV）、基孔肯雅热病毒（CHIKV）、寨卡病毒（ZIKV）、黄热病病毒（YFV）和西尼罗河病毒（WNV），是这些传播媒介蚊子流行地区的重要公共卫生关注点，但最近在欧洲也有来自这些流行地区的旅行者感染的病例报告。因此，对这些病毒的早期诊断对于患者管理和采取预防措施至关重要。本文描述了一种基于多重实时（RT）-PCR 的同时检测 DENV、CHIKV、ZIKV、YFV 和 WNV 的方法，及其在非流行国家的感染筛查和虫媒病毒监测中的诊断应用价值。
