Kajigaya S, Arakawa H, Kuge S, Koi T, Imura N, Nomoto A
Virology. 1985 Apr 30;142(2):307-16. doi: 10.1016/0042-6822(85)90339-3.
Defective-interfering (DI) particles of the Sabin strain of type 1 poliovirus were generated on serial high m.o.i. passaging. The deletions, measured by agarose gel electrophoresis, appeared to comprise approximately 10% of the total genome. Analysis of the RNAs, after digestion with RNase T1, by two-dimensional polyacrylamide gel electrophoresis revealed that the locations of the deleted genome regions were similar to those of the DI particles of the Mahoney strain of type 1 poliovirus (A. Nomoto, A. Jacobson, Y. F. Lee, J. Dunn, and E. Wimmer, (1979), J. Mol. Biol. 128, 179-196). Taking the known nucleotide sequences of the total genome and large RNase T1-resistant oligonucleotides into account, the deletions of almost all DI RNAs were found to exist between nucleotide positions 1307 and 2630, a genome region encoding capsid polypeptides VP2, VP3, and VP1. In cells coinfected with the purified DI particles and the Sabin strain of type 2 or type 3 poliovirus, particles containing the DI genomes were effectively produced. These results suggest that encapsidation signals are conserved in all three serotypes of polioviruses. However, only a very small amount of similar DI particles appeared to be produced in cells coinfected with coxsackie virus B1, although the genomes of polioviruses and coxsackie viruses have common sequences and therefore these viruses are considered to have arisen from a common ancestor. These data may suggest differences in encapsidation signals between polioviruses and coxsackie viruses.
通过连续高感染复数传代产生了1型脊髓灰质炎病毒萨宾株的缺陷干扰（DI）颗粒。经琼脂糖凝胶电泳测定，缺失部分约占基因组总量的10%。用核糖核酸酶T1消化后，通过二维聚丙烯酰胺凝胶电泳对RNA进行分析，结果显示，缺失的基因组区域位置与1型脊髓灰质炎病毒马奥尼株的DI颗粒相似（A.野本、A.雅各布森、Y.F.李、J.邓恩和E.维默，（1979年），《分子生物学杂志》128卷，第179 - 196页）。考虑到全基因组和大的核糖核酸酶T1抗性寡核苷酸的已知核苷酸序列，发现几乎所有DI RNA的缺失都存在于核苷酸位置1307和2630之间，该基因组区域编码衣壳多肽VP2、VP3和VP1。在同时感染纯化的DI颗粒和2型或3型脊髓灰质炎病毒萨宾株的细胞中，有效地产生了含有DI基因组的颗粒。这些结果表明，脊髓灰质炎病毒的所有三种血清型中衣壳化信号是保守的。然而，在同时感染柯萨奇病毒B1的细胞中，似乎只产生了极少量类似的DI颗粒，尽管脊髓灰质炎病毒和柯萨奇病毒的基因组有共同序列，因此这些病毒被认为起源于共同的祖先。这些数据可能表明脊髓灰质炎病毒和柯萨奇病毒在衣壳化信号上存在差异。
