脊髓灰质炎病毒RNA的序列研究。IV. 1型、2型脊髓灰质炎病毒及两种1型缺陷干扰颗粒RNA的核苷酸序列复杂性，以及3型脊髓灰质炎病毒基因组的指纹图谱。
Sequence studies of poliovirus RNA. IV. Nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles RNAs, and fingerprint of the poliovirus type 3 genome.
作者信息
Lee Y F, Kitamura N, Nomoto A, Wimmer E
出版信息
J Gen Virol. 1979 Aug;44(2):311-22. doi: 10.1099/0022-1317-44-2-311.
The 32P-labelled genomes of poliovirus type 1, 2 and 3 have been digested with RNase T1 and the products separated by two-dimensional gel electrophoresis. All three fingerprints differ in the separation pattern of the large oligonucleotides. The molar yields of the large RNase T1-resistant oligonucleotides of type 1 and type 2 RNA of poliovirus RNA are close to one. By comparing the yields of these oligonucleotides to the amount of RNA from which they originated, the chain length of type 1 poliovirus RNA was found to be 7851 +/- 567 nucleotides (mol. wt. 2.66 +/- 0.19 x 10(6) and that of poliovirus type 2, 8181 +/- 578 nucleotides (mol. wt. 2.77 +/- 0.19 x 10(6). The chain length of two defective interfering particle (DI) RNAs of poliovirus type 1 were determined to be 7042 +/- 999 nucleotides for DI(1) and 6639 +/- 674 nucleotides for DI(2).
1型、2型和3型脊髓灰质炎病毒的32P标记基因组已用核糖核酸酶T1消化，产物通过二维凝胶电泳分离。所有三种指纹图谱在大寡核苷酸的分离模式上都有所不同。脊髓灰质炎病毒RNA的1型和2型RNA的大核糖核酸酶T1抗性寡核苷酸的摩尔产量接近1。通过将这些寡核苷酸的产量与其来源的RNA量进行比较，发现1型脊髓灰质炎病毒RNA的链长为7851±567个核苷酸（分子量2.66±0.19×10⁶），2型脊髓灰质炎病毒的链长为8181±578个核苷酸（分子量2.77±0.19×10⁶）。1型脊髓灰质炎病毒的两种缺陷干扰颗粒（DI）RNA的链长测定为DI(1)为7042±999个核苷酸，DI(2)为6639±674个核苷酸。
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