Bell J R, Kinney R M, Trent D W, Lenches E M, Dalgarno L, Strauss J H
Virology. 1985 May;143(1):224-9. doi: 10.1016/0042-6822(85)90110-2.
N-terminal amino acid sequences of structural proteins of three flaviviruses, yellow fever, St. Louis encephalitis, and dengue-2 viruses, have been obtained. The glycoproteins of these three viruses are 52-60% conserved in the region sequenced, depending upon which pair of viruses are compared, and 40% of the amino acids are invariant in all three viruses. Thus, flaviviruses are closely related and have in all probability descended from a common ancestor. Furthermore, residues important in the secondary structure of proteins are conserved, suggesting that the overall conformation of the glycoproteins is the same in all three viruses while considerable variation in the primary sequence can be accommodated. The N-terminal regions of the nucleocapsid proteins of yellow fever and St. Louis encephalitis viruses show markedly less homology (25%) and this region is highly basic with one-quarter (yellow fever) or one-third (St. Louis encephalitis) of the residues being lysine or arginine. N-terminal sequences for the M protein of yellow fever and for NV2(GP19) of St. Louis encephalitis viruses are also reported.
已获得三种黄病毒（黄热病病毒、圣路易斯脑炎病毒和登革热2型病毒）结构蛋白的N端氨基酸序列。这三种病毒的糖蛋白在测序区域的保守性为52%-60%，具体取决于所比较的病毒对，并且在所有三种病毒中40%的氨基酸是不变的。因此，黄病毒密切相关，很可能起源于一个共同的祖先。此外，蛋白质二级结构中重要的残基是保守的，这表明所有三种病毒中糖蛋白的整体构象相同，同时一级序列中可以有相当大的变异。黄热病病毒和圣路易斯脑炎病毒核衣壳蛋白的N端区域显示出明显较低的同源性（25%），并且该区域具有高度碱性，四分之一（黄热病病毒）或三分之一（圣路易斯脑炎病毒）的残基为赖氨酸或精氨酸。还报道了黄热病病毒M蛋白和圣路易斯脑炎病毒NV2（GP19）的N端序列。
