Trent D W, Kinney R M, Johnson B J, Vorndam A V, Grant J A, Deubel V, Rice C M, Hahn C
Virology. 1987 Feb;156(2):293-304. doi: 10.1016/0042-6822(87)90409-0.
cDNA clones of the St. Louis encephalitis (SLE) virus genome have been obtained and the nucleotide sequence of 4.7 kb corresponding to the 5' terminal half of the genome determined. The genome contains a 5' noncoding region of 98 nucleotides followed by a single continuous open reading frame that encodes three structural proteins in the order capsid (C), membrane precursor (prM)-membrane (M), and envelope (E). Immediately following the C-terminus of E are located nonstructural proteins NS1 through NS3. The SLE amino acid sequence homology with yellow fever (YF), Murray Valley encephalitis (MVE), West Nile (WN), and dengue-2 (DEN) viruses over the sequenced region is 39, 66, 64, and 43%, respectively. The start of each SLE protein has been assigned on the basis of N-terminal sequence data and potential proteolytic cleavage sites homologous with YF and MVE viruses. Flaviviruses have conserved glycosylation sites in prM and NS1 proteins, although only one of the two glycosylation sites in the SLE E protein is conserved in MVE and DEN viruses. An evolutionary tree showing relationships of SLE, MVE, WN, YF, and DEN-2 flaviviruses is proposed on the basis of the amino acid sequences of the C proteins.
已获得圣路易斯脑炎（SLE）病毒基因组的cDNA克隆，并确定了对应于基因组5'末端一半的4.7kb核苷酸序列。该基因组包含一个98个核苷酸的5'非编码区，其后是一个单一的连续开放阅读框，该阅读框按衣壳（C）、膜前体（prM）-膜（M）和包膜（E）的顺序编码三种结构蛋白。在E的C末端之后紧接着是非结构蛋白NS1至NS3。在测序区域，SLE与黄热病（YF）、墨累谷脑炎（MVE）、西尼罗河（WN）和登革热-2（DEN）病毒的氨基酸序列同源性分别为39%、66%、64%和43%。根据N端序列数据以及与YF和MVE病毒同源的潜在蛋白水解切割位点，确定了每个SLE蛋白的起始位置。黄病毒在prM和NS1蛋白中具有保守的糖基化位点，尽管SLE E蛋白中的两个糖基化位点中只有一个在MVE和DEN病毒中是保守的。基于C蛋白的氨基酸序列，提出了一个显示SLE、MVE、WN、YF和DEN-2黄病毒之间关系的进化树。
