Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Fertil Steril. 2018 Jul 1;110(1):172-181.e4. doi: 10.1016/j.fertnstert.2018.03.007.
To regenerate functional endometrium tissue using "cell sheet" techniques as a regenerative medicine approach to address endometrial disorders causing female factor infertility.
In vivo experimental study.
Preclinical surgical and biomedical research laboratories.
ANIMAL(S): Green fluorescent protein (GFP) transgenic rats [SD-Tg (CAG-EGFP) rats] and nude rats (F344/NJcl-rnu/rnu).
INTERVENTION(S): GFP-positive rat uterine-derived cells as cell sheets were transplanted into resected rat uterine endometrial sites. Transplanted cell sheet areas were then analyzed using macroscopic observations and histological analysis including immunohistochemistry. Subsequently, crossbreeding was performed to establish fertility and confirm pregnancy in the rat-regenerated uterus.
MAIN OUTCOME MEASURE(S): Morphologic and biochemical markers of regenerated endometrium and establishment of pregnancy in otherwise sterile animals.
RESULT(S): After cell sheet transplantation, regenerated endometrium was confirmed as GFP-positive tissue engraftment both visually and under histological analysis. After crossbreeding, GFP-positive tissue areas and living fetuses were observed in the transplantation group.
CONCLUSION(S): Cell sheet transplantation can regenerate endometrial tissue with histological structure and physiological function supporting pregnancy similar to normal endometrial tissue. Translation of this endometrial cell sheet transplantation method to human patients with endometrial disorders could yield a novel therapy for uterine infertility.
利用“细胞片”技术再生功能性子宫内膜组织，作为一种再生医学方法，解决导致女性不孕的子宫内膜疾病。
体内实验研究。
临床前外科和生物医学研究实验室。
绿色荧光蛋白（GFP）转基因大鼠[SD-Tg（CAG-EGFP）大鼠]和裸鼠（F344/NJcl-rnu/rnu）。
作为细胞片的 GFP 阳性大鼠子宫来源细胞被移植到切除的大鼠子宫内膜部位。然后使用宏观观察和包括免疫组织化学在内的组织学分析来分析移植的细胞片区域。随后进行杂交以建立在再生子宫中的生育能力并确认妊娠。
再生子宫内膜的形态和生化标志物以及无菌动物的妊娠建立。
细胞片移植后，在视觉上和组织学分析中均证实再生的子宫内膜为 GFP 阳性组织移植物。杂交后，在移植组中观察到 GFP 阳性组织区域和活胎。
细胞片移植可以再生具有支持妊娠的组织学结构和生理功能的子宫内膜组织，类似于正常子宫内膜组织。将这种子宫内膜细胞片移植方法应用于子宫内膜疾病的人类患者可能为子宫不孕提供一种新的治疗方法。
