Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
Department of Otolaryngology-Head and Neck Surgery, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
Cells. 2020 Dec 9;9(12):2645. doi: 10.3390/cells9122645.
Thermoresponsive cell culture plates release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining functional extracellular matrix proteins and tight junctions, both of which indicate formation of intact and functional tissue. Our recent studies demonstrated that cell sheets are highly effective in promoting mouse submandibular gland (SMG) cell differentiation and recovering tissue integrity. However, these studies were performed only at early time points and extension of the observation period is needed to investigate duration of the cell sheets. Thus, the goal of this study was to demonstrate that treatment of wounded mouse SMG with cell sheets is capable of increasing salivary epithelial integrity over extended time periods. The results indicate that cell sheets promote tissue organization as early as eight days after transplantation and that these effects endure through Day 20. Furthermore, cell sheet transplantation in wounded SMG induces a significant time-dependent enhancement of cell polarization, differentiation and ion transporter expression. Finally, this treatment restored saliva quantity to pre-wounding levels at both eight and twenty days post-surgery and significantly improved saliva quality at twenty days post-surgery. These data indicate that cell sheets engineered with thermoresponsive cell culture plates are useful for salivary gland regeneration and provide evidence for the long-term stability of cell sheets, thereby offering a potential new therapeutic strategy for treating hyposalivation.
温敏型细胞培养板可使细胞在温度发生微小变化时作为融合的活细胞层释放出来，回收的细胞层保留了功能性细胞外基质蛋白和紧密连接，这两者都表明形成了完整且功能正常的组织。我们最近的研究表明，细胞片在促进小鼠下颌下腺 (SMG) 细胞分化和恢复组织完整性方面非常有效。然而，这些研究仅在早期时间点进行，需要延长观察期来研究细胞片的持续时间。因此，本研究的目的是证明用细胞片处理受伤的小鼠 SMG 能够在延长的时间内增加唾液上皮的完整性。结果表明，细胞片早在移植后 8 天就促进了组织的组织化，并且这些作用持续到第 20 天。此外，细胞片在受伤的 SMG 中的移植诱导了细胞极化、分化和离子转运蛋白表达的显著时间依赖性增强。最后，这种治疗在手术后 8 天和 20 天都将唾液量恢复到受伤前的水平，并在手术后 20 天显著改善了唾液质量。这些数据表明，使用温敏型细胞培养板构建的细胞片可用于唾液腺再生，并为细胞片的长期稳定性提供了证据，从而为治疗唾液过少提供了一种潜在的新治疗策略。
