Okamoto T, Josephs S F, Kawanishi M, Wong-Staal F
Virology. 1985 Jun;143(2):636-9. doi: 10.1016/0042-6822(85)90404-0.
The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine et al. (W. A. Haseltine, J. Sodroski, R. Patarca, D. Briggs, D. Perkins, and F. Wong-Staal, Science (Washington, D. C. 225, 421-424 (1984); K. Shimotono, W. Wachsman, Y. Takahashi, D. W. Golde, M. Miwa, T. Sigimura, and I. S. Y. Chen, Proc. Natl. Acad. Sci. USA 81, 6657-6661 (1984)) that a pX protein with a molecular weight of at least 38,000 could be synthesized. Generation of a 2.0-kb pX mRNA may involve a double-splicing event.
利用S1核酸酶保护分析法已确定，HTLV-I的pX基因的剪接受体位点位于碱基位置7301。尽管pX mRNA的丰度水平存在差异，但在所有研究的HTLV-I永生化T细胞克隆中都使用了这个剪接受体位点。我们的结果证实了哈泽尔廷等人（W. A. 哈泽尔廷、J. 索德罗斯基、R. 帕塔尔卡、D. 布里格斯、D. 珀金斯和F. 王-斯塔尔，《科学》（华盛顿特区）225, 421 - 424 (1984); K. 下友野、W. 瓦克斯曼、Y. 高桥、D. W. 戈尔德、M. 三泽、T. 志村和I. S. Y. 陈，《美国国家科学院院刊》81, 6657 - 6661 (1984)）的提议，即可以合成一种分子量至少为38,000的pX蛋白。2.0-kb pX mRNA的产生可能涉及双剪接事件。
