Josephs S F, Wong-Staal F, Manzari V, Gallo R C, Sodroski J G, Trus M D, Perkins D, Patarca R, Haseltine W A
Virology. 1984 Dec;139(2):340-5. doi: 10.1016/0042-6822(84)90379-9.
Variation in the structure of the long terminal repeat (LTR) element of human T-cell leukemia virus (HTLV) types has been noted (M. Seiki, S. Hattori, Y. Hirayama, and M. Yoshida (1983), Proc. Natl. Acad. Sci. USA 80, 3618-3622; K. Shimotohno, D. W. Golde, M. Miwa, T. Sugimura, and I. S. Y. Chen (1984), Proc. Natl. Acad. Sci. USA 81, 1079-1083; J. Sodroski, M. Trus, D. Perkins, R. Patarca, F. Wong-Staal, E. Gelmann, R. Gallo, and W. Haseltine (1984), Proc. Natl. Acad. Sci. USA 81, 4617-4621). To determine whether HTLV isolates with similar disease associations, but from different geographic locations, exhibit a conserved LTR structure, the nucleotide sequence of the LTR of an American HTLV isolate from a patient with adult T-cell leukemia/lymphoma was obtained. Comparison of this LTR sequence to that of two Japanese HTLV isolates associated with a similar disease reveals a highly conserved organization of the U3, R, and U5 regions. The U. S. isolate differs from the Japanese viruses by only 15-16 bases out of 754 bases in the LTR region. These results show that Japanese HTLV isolates from patients with adult T-cell leukemia/lymphoma are members of the HTLV-I family and that LTRs of HTLV-I isolates are highly conserved. A 50-nucleotide imperfect direct repeat element is also identified in the U3 of the HTLV LTR distant from the cap site. The position and conserved nature of this sequence make it a likely candidate for a transcriptional enhancer.
人们已经注意到人类T细胞白血病病毒（HTLV）各型长末端重复序列（LTR）元件结构的差异（M. 关木、S. 服部、Y. 平山和M. 吉田（1983年），《美国国家科学院院刊》80，3618 - 3622；K. 下户野、D. W. 戈尔德、M. 三泽、T. 杉村和I. S. Y. 陈（1984年），《美国国家科学院院刊》81，1079 - 1083；J. 索德罗斯基、M. 特鲁斯、D. 珀金斯、R. 帕塔尔卡、F. 王 - 斯塔尔、E. 格尔曼、R. 加洛和W. 哈泽尔廷（1984年），《美国国家科学院院刊》81，4617 - 4621）。为了确定来自不同地理位置但具有相似疾病关联的HTLV分离株是否具有保守的LTR结构，我们获得了一名成人T细胞白血病/淋巴瘤患者的美国HTLV分离株LTR的核苷酸序列。将该LTR序列与两种与类似疾病相关的日本HTLV分离株的序列进行比较，发现U3、R和U5区域具有高度保守的组织架构。美国分离株与日本病毒在LTR区域的754个碱基中仅相差15 - 16个碱基。这些结果表明，来自成人T细胞白血病/淋巴瘤患者的日本HTLV分离株是HTLV - I家族的成员，并且HTLV - I分离株的LTR高度保守。在远离帽位点的HTLV LTR的U3中还鉴定出一个50个核苷酸的不完全直接重复元件。该序列的位置和保守性质使其成为转录增强子的可能候选者。
