Fuss P, Bal K, Jerzyńska J, Podlecka D, Stelmach W, Stelmach I
Department of Allergology and Internal Medicine in children, Copernicus Hospital, al. Pilsudskiego 71, 90-329, Lodz, Poland.
Department of Social and Preventive Medicine, Medical University of Lodz, Lodz, Poland.
Allergol Immunopathol (Madr). 2019 Jan-Feb;47(1):43-46. doi: 10.1016/j.aller.2018.04.003. Epub 2018 Jul 3.
It is considered that farm areas protect young patients from allergy and asthma due to high exposure to endotoxins.
To compare CD4+/CD25+ T-regulatory cells and forkhead transcription factor Foxp3 expression in asthmatic children allergic to house dust mites (HDM) living in rural and farm areas.
This was a prospective analysis of 35 children living in farm areas (n=19) and rural areas (n=16), aged 8-16, with allergic rhinitis (allergic to dust mites) and newly diagnosed asthma. Surface molecule CD4+CD25+Foxp3+ expression on cultured PBMCs was estimated by flow cytometry using fluorophore-conjugated monoclonal antibodies in each patient.
Thirty-five children were included into the analysis: 19 children living in farm areas and 16 in rural areas. Within and between-groups (farm area vs. rural area) differences in CD4+/CD25+ and CD4+/CD25+Foxp3+ cell expression did not reach the level of significance.
The current analysis showed that CD4+/CD25+ and CD4+/CD25+Foxp3+ cell expression was not associated with place of living in asthmatic children sensitive to HDM.
由于高暴露于内毒素，农村地区被认为可保护年轻患者免受过敏和哮喘的影响。
比较生活在农村和农场地区对屋尘螨（HDM）过敏的哮喘儿童中CD4+/CD25+调节性T细胞和叉头转录因子Foxp3的表达。
这是一项对35名年龄在8 - 16岁、患有过敏性鼻炎（对尘螨过敏）且新诊断为哮喘的农村地区儿童（n = 19）和农场地区儿童（n = 16）进行的前瞻性分析。使用荧光团偶联的单克隆抗体通过流式细胞术估计每位患者培养的外周血单核细胞（PBMC）上的表面分子CD4+CD25+Foxp3+表达。
35名儿童纳入分析：19名生活在农场地区，16名生活在农村地区。组内和组间（农场地区与农村地区）CD4+/CD25+和CD4+/CD25+Foxp3+细胞表达的差异未达到显著水平。
当前分析表明，对于对HDM敏感的哮喘儿童，CD4+/CD25+和CD4+/CD25+Foxp3+细胞表达与居住地点无关。
