Weiner D, Gibson W, Fields K L
Virology. 1985 Nov;147(1):19-28. doi: 10.1016/0042-6822(85)90223-5.
A monospecific, polyclonal antiserum to the 69-kDa matrix protein of human cytomegalovirus (HCMV) was prepared in a guinea pig and used to determine the intracellular distribution of this viral antigen. The resulting antiserum was specific for infected cells as tested by immunofluorescence, and specific for the HCMV matrix protein as determined by "nitrocellulose immunoassay" of electrophoretically separated, infected-cell proteins. Antibodies were reacted with fixed, infected human fibroblasts, and visualized by the anti-complement immunofluorescence procedure to avoid complications arising from the strong IgG Fc binding activity of the infected-cell-specific cytoplasmic inclusion. Results establish that the matrix protein is located in the nucleus, and indicate that it is concentrated in the nucleoplasm rather than within the intranuclear inclusions.
用人巨细胞病毒（HCMV）的69-kDa基质蛋白制备了单特异性多克隆抗血清，并将其用于豚鼠体内，以确定该病毒抗原的细胞内分布。通过免疫荧光检测，所得抗血清对感染细胞具有特异性；通过对经电泳分离的感染细胞蛋白进行“硝酸纤维素免疫测定”，确定其对HCMV基质蛋白具有特异性。将抗体与固定的感染人成纤维细胞反应，并通过抗补体免疫荧光法进行可视化，以避免因感染细胞特异性胞质内含物的强IgG Fc结合活性而产生的并发症。结果证实基质蛋白位于细胞核中，并表明它集中在核质中，而不是核内包涵体中。
