Peluso R, Haase A, Stowring L, Edwards M, Ventura P
Virology. 1985 Nov;147(1):231-6. doi: 10.1016/0042-6822(85)90246-6.
Visna virus is the prototype of the lentivirus subfamily of retroviruses that cause slow infections of sheep and goats. These viruses persist and can be isolated from blood and cerebrospinal fluid for years despite neutralizing antibody. In the studies reported here we have used quantitative in situ hybridization to analyze infected leukocytes. We show that (1) monocytes harbor the visna genome; and (2) virus gene expression is as constrained in this cell as it is in glial and epithelial cells. These results are in accord with a Trojan Horse mechanism of virus dissemination in an immunologically responsive host.
维斯纳病毒是逆转录病毒慢病毒亚科的原型，可引起绵羊和山羊的慢性感染。尽管存在中和抗体，这些病毒仍会持续存在，并可从血液和脑脊液中分离数年。在本文报道的研究中，我们使用定量原位杂交技术分析受感染的白细胞。我们发现：（1）单核细胞携带着维斯纳病毒基因组；（2）该病毒在这种细胞中的基因表达与在神经胶质细胞和上皮细胞中一样受到限制。这些结果与病毒在免疫反应性宿主中传播的特洛伊木马机制相一致。
