Quambusch Mona, Winkelmann Traud
Abteilung Waldgenressourcen, Nordwestdeutsche Forstliche Versuchsanstalt, Hann. Münden, Germany.
Institut für Gartenbauliche Produktionssysteme, Leibniz Universität Hannover, Hannover, Germany.
Methods Mol Biol. 2018;1815:69-88. doi: 10.1007/978-1-4939-8594-4_4.
Endophytic bacteria have been increasingly in the focus of research projects during the last decade. This has changed the view on bacteria in plant tissue culture and led to the differentiation between artificially introduced contaminations and naturally occurring endophytes with neutral, negative, or positive impact on the plant propagation process. This review chapter gives an overview on recent findings about the impact that bacteria have on the plant physiology in general and during micropropagation. Additionally, methods for the detection and identification of bacteria in plant tissue are described and, finally, suggestions of how to deal with bacterial endophytes in in vitro culture are given.
在过去十年中,内生细菌越来越成为研究项目的焦点。这改变了人们对植物组织培养中细菌的看法,并导致了对人工引入的污染物和对植物繁殖过程有中性、负面或正面影响的天然内生菌的区分。本章综述了关于细菌对植物生理总体影响以及在微繁殖过程中影响的最新研究结果。此外,还描述了检测和鉴定植物组织中细菌的方法,最后给出了在体外培养中如何处理细菌内生菌的建议。