Castro-Restrepo Dagoberto, Dominguez Maria Isabel, Gaviria-Gutiérrez Bertha, Osorio Edison, Sierra Karina
Unidad de Biotecnología Vegetal, Facultad de Ciencias Agropecuarias, Universidad Católica de Oriente, Cr46-40 B-50, Rionegro 054040, Colombia.
Grupo de Investigación Sanidad Vegetal, Facultad de Ciencias Agropecuarias, Universidad Católica de Oriente, Rionegro 054040, Colombia.
Plants (Basel). 2022 May 31;11(11):1474. doi: 10.3390/plants11111474.
In the present study, the effect of biotization of microplants with and on growth, sp., incidence, and specialized metabolites content was evaluated. Analyses of root tissues of the microplants showed 100% endophytism with both microorganisms. During the acclimatization phase, plants with the endophytes and had a survival rate of 95% and 93%, respectively, compared to 75% for control plants. Then, under greenhouse conditions, a trial was carried out with biotized plants with or without sp. inoculation, plants inoculated with sp., and endophyte- and pathogen-free control. Biotized plants with the endophytes showed higher dry biomass and the incidence of was lower (8% for and 10% for ) compared to plants inoculated with the pathogen (82%). In addition, plants with had the highest contents of total polyphenols (280 GAE/100 mg sample) and rosmarinic acid (28 mg RA/100 g sample). Thus, this study shows the potential of the technique of using the endophytes and on microplants to improve plant survival and growth, decrease the incidence of sp., and improve the contents of specialized metabolites, which can contribute to the sustainable management of this crop.
在本研究中，评估了用[具体微生物1]和[具体微生物2]对微型植物进行生物处理对其生长、[病害名称]发病率及次生代谢产物含量的影响。对微型植物的根组织分析表明，这两种微生物的内生定殖率均为100%。在驯化阶段，带有内生菌[具体微生物1]和[具体微生物2]的植物存活率分别为95%和93%，而对照植物的存活率为75%。然后，在温室条件下，对经生物处理的植物进行了试验，这些植物接种或未接种[病害名称]病原菌，还有接种了[病害名称]病原菌的植物以及无内生菌和病原菌的对照植物。与接种病原菌的植物（82%）相比，带有内生菌的生物处理植物表现出更高的干生物量，且[病害名称]发病率更低（[具体微生物1]处理的为8%，[具体微生物2]处理的为10%）。此外，带有[具体微生物2]的植物总多酚含量最高（280 GAE/100 mg样品），迷迭香酸含量也最高（28 mg RA/100 g样品）。因此，本研究表明利用内生菌[具体微生物1]和[具体微生物2]对微型植物进行处理的技术具有提高植物存活率和生长、降低[病害名称]病原菌发病率以及提高次生代谢产物含量的潜力，这有助于该作物的可持续管理。
