Liu Yunxia, Cheng Yuqiang, Shan Wenya, Ma Jingjiao, Wang Hengan, Sun Jianhe, Yan Yaxian
Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Dev Comp Immunol. 2018 Nov;88:77-82. doi: 10.1016/j.dci.2018.07.003. Epub 2018 Jul 5.
Interferon regulatory factors (IRFs) is an important family for IFN expression regulating while viral infection. IRF1, IRF3, and IRF7 are the primary regulators that trigger type I IFN response in mammals. However, IRF3, which has been identified as the most critical regulator in mammals, is absent in chickens, and it is unknown whether IRF1 is involved in type I IFN signaling pathways in IRF3-deficient chicken cells. Here, we identified chicken IRF1 (chIRF1) as a critical IFN-β mediator in response to viral infection. Overexpression of chIRF1 activated IFN-β intensively and suppressed AIV and NDV viral replication. Moreover, the mRNA levels of IFN-β and ISGs increased during chIRF1 overexpression. In addition, deletion mutant analysis revealed that the first four domains of chIRF1 are indispensable for IFN-β induction. Together, our studies demonstrate that chIRF1 is an important regulator of IFN-β and is involved in chicken antiviral innate immunity.
干扰素调节因子（IRFs）是病毒感染期间调节IFN表达的一个重要家族。IRF1、IRF3和IRF7是在哺乳动物中触发I型IFN反应的主要调节因子。然而，已被确定为哺乳动物中最关键调节因子的IRF3在鸡中不存在，并且尚不清楚IRF1是否参与IRF3缺陷鸡细胞中的I型IFN信号通路。在此，我们鉴定出鸡IRF1（chIRF1）是病毒感染时关键的IFN-β介导因子。chIRF1的过表达强烈激活IFN-β并抑制禽流感病毒（AIV）和新城疫病毒（NDV）的复制。此外，在chIRF1过表达期间，IFN-β和干扰素刺激基因（ISGs）的mRNA水平增加。另外，缺失突变分析表明chIRF1的前四个结构域对于IFN-β诱导是不可或缺的。总之，我们的研究表明chIRF1是IFN-β的重要调节因子，并参与鸡的抗病毒天然免疫。
