Sharifi-Rad Mehdi, Salehi Bahar, Sharifi-Rad Javad, Setzer William N, Iriti Marcello
Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran.
Medical Ethics and Law Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Mol Biol (Noisy-le-grand). 2018 Jun 25;64(8):18-21.
Leishmaniasis is a neglected parasitic protozoal disease that affects approximately 12 million people and represents a public health problem in Iran. The objectives of this study were to obtain the essential oil (EO) from Pulicaria vulgaris Gaertn. growing in Iran and to carry out in-vitro antileishmanial screening of the EO against promastigotes of Leishmania major and Leishmania infantum. The EO from the aerial parts of P. vulgaris was extracted by hydrodistillation. Serial dilutions of the EO were screened for in-vitro antileishmanial activity using 96-well microtiter plates. The P. vulgaris EO was active against the promastigote forms of L. major and L. infantum, with IC50 values of 244.70 and 233.65 µg/mL, respectively. Pulicaria vulgaris EO may serve as an alternative or complementary treatment for leishmaniasis.
利什曼病是一种被忽视的寄生虫原生动物疾病，影响着约1200万人，在伊朗是一个公共卫生问题。本研究的目的是从生长在伊朗的普通刺菜（Pulicaria vulgaris Gaertn.）中获取精油（EO），并对该精油针对硕大利什曼原虫（Leishmania major）和婴儿利什曼原虫（Leishmania infantum）前鞭毛体进行体外抗利什曼原虫筛选。普通刺菜地上部分的精油通过水蒸馏法提取。使用96孔微量滴定板对精油的系列稀释液进行体外抗利什曼原虫活性筛选。普通刺菜精油对硕大利什曼原虫和婴儿利什曼原虫的前鞭毛体形式具有活性，IC50值分别为244.70和233.65μg/mL。普通刺菜精油可作为利什曼病的替代或补充治疗方法。
